Mechanism and Characterization of Polyamide 4 Degradation by <Emphasis Type="Italic">Pseudomonas</Emphasis> sp. |
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Authors: | Naoko Yamano Atsuyoshi Nakayama Norioki Kawasaki Noboru Yamamoto Seiichi Aiba |
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Institution: | (1) Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), 1-8-31, Midorigaoka, Ikeda Osaka, 563-8577, Japan |
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Abstract: | We identified a biodegrading microorganism of polyamide (nylon) 4, a linear polymer of γ-aminobutyric acid (GABA). From activated
sludge, the biodegrading bacteria strains of Pseudomonas sp. were isolated and identified by their taxonomic characteristics and nucleotide sequences of 16S rDNA. One strain, ND-11,
was grown on a minimal medium containing polyamide 4 (PA4) as the sole carbon source. The strain produced GABA as a degradation
intermediate, as identified by analyzing the NMR spectra of degraded products. The culture supernatant of strain ND-11 degraded
the emulsified PA4 completely within one day. These results suggest that the ND-11 strain degraded PA4 using its extracellular
enzymes to hydrolyze amide bonds. |
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Keywords: | Polyamide Nylon Biodegradation Degrading bacteria Activated sludge γ -Aminobutyric acid |
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