Characterization and enzymatic degradation of a cross-linked bacterial polyester |
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Authors: | Thomas M Scherer R Clinton Fuller Robert W Lenz |
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Institution: | (1) Department of Polymer Science and Engineering, University of Massachusetts, 01003 Amherst, Massachusetts;(2) Department of Biochemistry and Molecular Biology, University of Massachusetts, 01003 Amherst, Massachusetts |
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Abstract: | The bacterial polyester, poly(-hydroxybutyrate-co--hydroxyvalerate) (PHB/V), was cross-linked with 1, 5, 7, 10, 20, and 30 wt% benzoyl peroxide by thermal decomposition reactions. Solvent extractions were carried out to determine the cross-linked fractions of the films. The sol/gel data were used to estimate cross-link densities. Films of PHB/V cross-linked with 10% benzoyl peroxide were placed in contact with purified depolymerase A secreted byP. lemoignei. These samples exhibited weight loss rates which were half that of un-cross-linked PHB/V, but the network was degraded completely by the enzyme. The results of this study suggest that anendo-type enzymatic degradation may occur, in addition to theexo-type activity, which is normally presumed to occur with theP. lemoignei depolymerase system. |
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Keywords: | Cross-linked poly(-hydroxybutyrate-co-gif" alt="beta" align="MIDDLE" BORDER="0">-hydroxybutyrate-co--hydroxyvalerate" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-hydroxyvalerate Pseudomonas lemoingei enzymatic degradation of poly(-hydroxyalkanoates)" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-hydroxyalkanoates) |
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