New bacterial strain of the genus Ochrobactrum with glyphosate-degrading activity |
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Authors: | Faranak Hadi Amir Mousavi Kambiz Akbari Noghabi Hadi Ghaderi Tabar Ali Hatef Salmanian |
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Institution: | 1. Department of Plant Biotechnology , National Institute of Genetic Engineering and Biotechnology (NIGEB) , Tehran , Iran;2. Department of Biology, Faculty of Science , Lorestan University , Khoramabad , Iran;3. Department of Plant Biotechnology , National Institute of Genetic Engineering and Biotechnology (NIGEB) , Tehran , Iran |
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Abstract: | Thirty bacterial strains with various abilities to utilize glyphosate as the sole phosphorus source were isolated from farm soils using the glyphosate enrichment cultivation technique. Among them, a strain showing a remarkable glyphosate-degrading activity was identified by biochemical features and 16S rRNA sequence analysis as Ochrobactrum sp. (GDOS). Herbicide (3 mM) degradation was induced by phosphate starvation, and was completed within 60 h. Aminomethylphosphonic acid was detected in the exhausted medium, suggesting glyphosate oxidoreductase as the enzyme responsible for herbicide breakdown. As it grew even in the presence of glyphosate concentrations as high as 200 mM, Ochrobactrum sp. could be used for bioremediation purposes and treatment of heavily contaminated soils. |
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Keywords: | Herbicide glyphosate bioremediation Ochrobactrum soils |
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