Biodegradation of Chlorimuron-ethyl by the Bacterium Klebsiella jilinsis 2N3 |
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Authors: | Hao Zhang Xianghui Zhang Wenhui Mu Jiaxiu Wang Hongyu Pan Yu Li |
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Institution: | 1. College of Resource and Environment , Jilin Agricultural University , Changchun, People's Republic of China;2. College of Plant Sciences , Jilin University , Changchun, People's Republic of China;3. Institute of Mycology, Jilin Agricultural University , Changchun, People's Republic of China |
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Abstract: | Enrichment culturing of sludge taken from an industrial wastewater treatment pond led to the identification of a bacterium (Klebsiella jilinsis H. Zhang) that degrades chlorimuron-ethyl with high efficiency. Klebsiella jilinsis strain 2N3 grows with chlorimuron-ethyl as the sole nitrogen source at the optimal temperature range of 30–35°C and pH values between 6.0–7.0. In liquid medium, the degradation activity was further induced by chlorimuron-ethyl. Degradation rates followed the pesticide degradation kinetic equation at concentrations between 20 and 200 mg L?1. Using initial concentrations of 20 and 100 mg L?1, the degradation rates of chlorimuron-ethyl were 83.5 % and 92.5 % in 12 hours, respectively. At an initial concentration higher than 200 mg L?1, the degradation rate decreased slightly as the concentration increased. The 2N3 strain also degraded the sulfonylurea herbicides ethametsulfuron, metsulfuron-methyl, nicosulfuron, rimsulfuron, and tribenuron-methyl. This study provides scientific evidence and support for the application of K. jilinsis in bioremediation to reduce environmental pollution. |
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Keywords: | Biodegradation chlorimuron-ethyl Klebsiella 16S rDNA phylogenetic analysis |
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