Bioremediation model for atrazine contaminated agricultural soils using phytoremediation (using Phaseolus vulgaris L.) and a locally adapted microbial consortium |
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| Authors: | Alfredo Madariaga-Navarrete Blanca Rosa Rodríguez-Pastrana José Roberto Villagómez-Ibarra Otilio Arturo Acevedo-Sandoval Gregory Perry |
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| Institution: | 1. Institute of Agricultural Sciences, Agronomy and Forestry Area, Universidad Autónoma del Estado de Hidalgo, Tulancingo, Hidalgo, Mexico;2. Academic Area of Chemistry, Institute of Basic Science and Engineering, Universidad Autónoma del Estado de Hidalgo, Pachuca, Hidalgo, Mexico;3. Department of Plant Agriculture, Ontario Agricultural College, University of Guelph, Guelph, Ontario, Canada |
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| Abstract: | The objective of the present study was to examine a biological model under greenhouse conditions for the bioremediation of atrazine contaminated soils. The model consisted in a combination of phytoremediation (using Phaseolus vulgaris L.) and rhizopheric bio-augmentation using native Trichoderma sp., and Rhizobium sp. microorganisms that showed no inhibitory growth at 10,000 mg L?1 of herbicide concentration. 33.3 mg of atrazine 50 g?1 of soil of initial concentration was used and an initial inoculation of 1 × 109 UFC mL?1 of Rhizobium sp. and 1 × 105 conidia mL?1 of Trichoderma sp. were set. Four treatments were arranged: Bean + Trichoderma sp. (B+T); Bean + Rhizobium sp. (BR); Bean + Rhizobium sp. + Trichoderma sp. (B+R+T) and Bean (B). 25.51 mg of atrazine 50 g?1 of soil (76.63%) was removed by the B+T treatment in 40 days (a = 0.050, Tukey). This last indicate that the proposed biological model and methodology developed is useful for atrazine contaminated bioremediation agricultural soils, which can contribute to reduce the effects of agrochemical abuse. |
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| Keywords: | Atrazine degradation locally adapted microorganism consortium bioremediation phytoremediation |
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