| 耐铅微生物的筛选及其吸附能力的初步研究 |
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| 引用本文： | 王俊丽,王忠,任建国.耐铅微生物的筛选及其吸附能力的初步研究[J].污染防治技术,2010,23(1):15-17,63. |
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| 作者姓名： | 王俊丽 王忠 任建国 |
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| 作者单位： | [1]青岛农业大学资源与环境学院,山东青岛266109 [2]青岛农业大学农学与植保学院,山东青岛266109 |
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| 基金项目： | 高校高层次人才启动基金(630724) |
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| 摘 要： | 通过向土壤中添加一定量的铅来驯化土壤微生物，然后利用一系列不同铅浓度的PDA培养基分离耐铅微生物，结果获得一株耐300mg／L铅的细菌Pb—R-1和二株分别耐750mtg／L和900mg／L铅的真菌Pb—R-2和Pb—R-3，通过鉴定得知，Pb—R-2菌株为曲霉，Pb—R-3菌株为青霉；不同成分培养基（有铅或无铅）传代培养实验表明，3个菌株有稳定的耐铅遗传性；通过液体悬浮培养获得：Pb—R-1、Pb—R-2和Pb—R-3对Pb^2＋的吸附率依次为35．5％、75．5％和48．2％。采用3个菌株浸种试验表明，小麦的发芽率几乎不受影响，其它的受影响差别较大。因此，用Pb—R-2吸附小麦土壤中的铅，具有潜在的应用价值。
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| 关 键 词： | 铅 微生物 筛选 吸附能力 |
Preliminary Study on Screening of Lead-Tolerant Microbes and Its Adsorption Capability |
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| WANG Jun-li,WANG Zhong,REN Jian-guo.Preliminary Study on Screening of Lead-Tolerant Microbes and Its Adsorption Capability[J].Pollution Control Technology,2010,23(1):15-17,63. |
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| Authors: | WANG Jun-li WANG Zhong REN Jian-guo |
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| Institution: | WANG Jun-li1,WANG Zhong1,REN Jian-guo2(1.College of Resources , Environment,QAU,Qingdao,Shangdong 266109,China,2.College of Agronomy , Plant Protection,China) |
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| Abstract: | The microorganisms was domesticated by adding a certain amount of lead in the soil,and three lead-tolerant strains including Pb-R-1(bacterium),Pb-R-2(fungus) and Pb-R-3(fungus) accordingly originating from 300 mg/L,750 mg/L and 900 mg/L lead medium were isolated with a series of PDA medium of different lead concentrations.Through the identification,Pb-R-2 was confirmed as Aspergillus sp.,Pb-R-3 as Penicillium sp.The trials of transferring culture showed that all strains had the stable heredity of lead toler... |
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| Keywords: | lead microorganism screen adsorption capability |
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