| 正十六烷微生物吸附降解的荧光显微研究 |
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| 引用本文： | 张小啸,刘连友,孙宁宁,王红旗,孙一娜.正十六烷微生物吸附降解的荧光显微研究[J].污染防治技术,2010,23(2):1-4. |
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| 作者姓名： | 张小啸 刘连友 孙宁宁 王红旗 孙一娜 |
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| 作者单位： | [1]民政部、教育部减灾与应急管理研究院,北京师范大学地表过程与资源生态国家重点实验室,环境演变与自然灾害教育部重点实验室,北京100875 [2]北京师范大学水科学研究院,北京100875 [3]北京师范大学细胞增殖及调控生物学教育部重点实验室,北京100875 |
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| 基金项目： | 国家自然科学基金资助项目,40771021; 科技部“十一五”支撑项目,2006BAD20B02 |
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| 摘 要： | 使用激光扫描共聚焦显微镜（CLSM）系统，以罗丹明123（Rhodamine123）为荧光探针，定量研究了石油组分正十六烷在石油降解菌（大庆油田石油污染土壤中的优势菌种，革兰氏阴性G^-、黄杆菌属Flavobacterium）细胞膜上的吸附降解动态变化过程。研究发现：微生物培养体系加入正十六烷120s后，正十六烷在菌株膜上的浓度变化在不断增大，呈现出不规则的锯齿状攀升；在攀升过程中，峰谷之间出现明显有“节奏”的振荡传递；在0～508内，正十六烷处于吸附运输的加速阶段；在50—120s内，正十六烷不断被吸附并累积于细胞膜表面，处于吸附积累阶段，使用幂指数定律建立该微生物膜上的正十六烷的动力学方程，确定其动力学级数为2级，微生物降解正十六烷的速率常数为6×e^-5。
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| 关 键 词： | 石油 降解菌 荧光探针 正十六烷 土壤 |
Fluorescent Microscopic Observation on Dynamic Degradation of N-Hexadecane by Soil Microbial Film |
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| ZHANG Xiao-xiao,LIU Lian-you,SUN Ning-ning,WANG Hong-qi,SUN Yi-na ,Key Laboratory of Environmental Change , Natural Disaster of Ministry of Education,Beijing Normal University,Beijing ,China.Fluorescent Microscopic Observation on Dynamic Degradation of N-Hexadecane by Soil Microbial Film[J].Pollution Control Technology,2010,23(2):1-4. |
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| Authors: | ZHANG Xiao-xiao LIU Lian-you SUN Ning-ning WANG Hong-qi SUN Yi-na Key Laboratory of Environmental Change Natural Disaster of Ministry of Education Beijing Normal University Beijing China |
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| Institution: | ZHANG Xiao-xiao1,LIU Lian-you1,SUN Ning-ning2,WANG Hong-qi2,SUN Yi-na3 (1.Academy of Disaster Reduction , Emergency Management,Ministry of Civil Affairs & Ministry of Education,State Key Laboratory of Earth Surface Processes , Resource Ecology(Beijing Normal University),Key Laboratory of Environmental Change , Natural Disaster of Ministry of Education,Beijing Normal University,Beijing 100875,China,2.Institute of Water Sciences,3.MOE Key Laboratory for Cel... |
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| Abstract: | This paper selected n-Hexadecane as representative oil pollutant in soil and used the microorganism(G-,Flavobacterium) from daqing oil fields(Heilongjiang Province,China),to observe and analyze its biodegradation process by CLSM(Confocal Laser Scanning Microscope).The Result showed that in 120 seconds,concentration(represented by Rhodamine123 as fluorescent probe) of n-Hexadecane absorbed on the microbial film increased in an irregular serration,and its peak value increased rhythmically during oscillating t... |
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| Keywords: | oil biodegradable bacteria fluorescent probe n-Hexadecane soil |
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