利用亚硝酸盐为电子受体反硝化聚磷菌的筛选与富集

依据DPB原理,利用SBR动态反应器和静态释/聚磷装置.以A2/O工艺厌氧段污泥为种泥,研究以亚硝酸盐为电子受体反硝化聚磷菌的筛选与富集,同时对选择、富集污泥的反硝化聚磷性能进行了考察.结果表明:利用亚硝酸盐为电子受体的反硝化聚磷菌存在于A2/O厌氧段污泥中,通过厌氧/好氧和厌氧/缺氧方式运行后,聚磷菌总数由1400个/mL增加到32 000个/mL,其中反硝化聚磷菌占聚磷菌总数的比例也由14.5%提高到81%,磷酸盐和亚硝酸盐去除率分别由最初的8.65%和7.55%上升到91%和95.62%;筛选与富集利用亚硝酸盐为电子受体的反硝化聚磷菌时,缺氧段进水COD的浓度须控制在10 mg/L以下;当体系处于稳定状态,且亚硝酸盐氮浓度高达30 mg/L时,并未对反硝化聚磷菌的生存产生抑制和体系运行产生干扰,此时磷酸盐出水低至1.06 mg/L.

Screening and enrichment of denitrifying phosphorus accumulating bacteria using nitrite as electronic acceptor

In this paper,according as the denitrifying phosphorus bacteria(DPB) principle,dynamic SBR reactor and static releasing/accumulating phosphorus installation were investigated.The anoxic sludge of A2/O technology was used as inoculum.The screening and enrichment of denitrifying phosphorus accumulating bacteria(DPB) using nitrite as electronic acceptor and its characteristics were studied.The result shows: the DPB using nitrite as electronic acceptor indeed lies in the anoxic sludge of A2/O technology.After anaerobic/aerobic and anaerobic/anoxic stages,the amount of PAO increases from 1400 to 32 000.The ratio of DPB to PAO improves from 14.5% to 81%.The removal rates of phosphate and nitrite rise from 8.65% and 7.55% to 91% and 95.62% respectively;when the DPB using nitrite as electronic acceptor is screened and enriched,the COD concentration of influent in the anoxic stage must be controlled below 10 mg/L.The existence of DPB is not restrained and the system is not disturbed when the system is stable and the nitrite concentration reaches as high as 30 mg/L.At the same time the concentration of phosphate in the effluent is below 1.06 mg/L.