| 生活污水中蛋白质测试方法研究 |
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| 引用本文： | 黄满红,李咏梅,顾国维.生活污水中蛋白质测试方法研究[J].环境工程学报,2009,3(3):417-421. |
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| 作者姓名： | 黄满红 李咏梅 顾国维 |
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| 作者单位： | 1. 东华大学环境科学与工程学院,上海,201620;同济大学环境科学与工程学院国家污染控制与资源化重点实验室,上海,200092
2. 同济大学环境科学与工程学院国家污染控制与资源化重点实验室,上海,200092 |
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| 基金项目： | 国家自然科学基金，东华大学校基金，上海市重点学科建设项目 |
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| 摘 要： | 分别用Folin-酚改进法、考马斯亮兰法和紫外分光光度法等3种测试方法测试了上海市某小区生活污水中的蛋白质含量,对这3种测试方法进行了比较,并采用氨基酸仪分析了生活污水中蛋白质水解后的氨基酸化学组成.试验结果表明,Folin-酚改进法测试蛋白质浓度的准确性与精确性优于考马斯亮兰法和紫外分光光度法,可用于测定生活污水中蛋白质的浓度;氨基酸仪法测得该生活污水中含有17种氨基酸,其中最多的为谷氨酸(3.6 mg/L),含量最低的是脯氨酸(0.8 mg/L);所测生活污水中蛋白质COD占总COD的18.9%.
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| 关 键 词： | 生活污水 蛋白质 Folin-酚改进法 考马斯亮兰法 紫外分光光度法 氨基酸 |
Study on determination methods of proteins in domestic wastewater |
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| Huang Manhong,Li Yongmei and Gu Guowei.Study on determination methods of proteins in domestic wastewater[J].Techniques and Equipment for Environmental Pollution Control,2009,3(3):417-421. |
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| Authors: | Huang Manhong Li Yongmei and Gu Guowei |
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| Institution: | 1.College of Environmental Science and Engineering;Donghua University;Shanghai 201620;2.State Key Laboratory of Pollution Control and Resources Reuse;College of Environmental Science and Engineering;Tongji University;Shanghai 200092 |
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| Abstract: | Proteins in domestic wastewater were measured by three methods(modified Folin-phenol method,Bradford method and spectrophotometer method).The chemical composition of proteins in domestic wastewater were also investigated with amino acids analyzer.The results showed that the Folin-phenol method was a more sensitive and accurate method than Bradford method and spectrophotometer method in determining proteins in domestic sewage.The wastewater was analyzed by amino acids analyzer after being hydrolyzed to amino... |
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| Keywords: | domestic wastewater proteins modified Folin-phenol method Bradford method spectrophotometer method amino acids |
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