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| 高效液相色谱法检测水中5种微囊藻毒素 | |
| 引用本文： | 毛敬英,杨敏,狄一安,曹金玲,任立军,许其功,席北斗.高效液相色谱法检测水中5种微囊藻毒素[J].环境工程学报,2012,6(11):3882-3888. |
| 作者姓名： | 毛敬英 杨敏 狄一安 曹金玲 任立军 许其功 席北斗 |
| 作者单位： | 1. 西南交通大学地球科学与环境工程学院,成都610031 中日环境友好保护中心,北京100029 中国环境科学研究院水环境系统工程研究室,北京100012 2. 西南交通大学地球科学与环境工程学院,成都,610031 3. 中日环境友好保护中心,北京,100029 4. 中国环境科学研究院水环境系统工程研究室,北京,100012 5. 中国环境科学研究院水环境系统工程研究室,北京100012 中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 |
| 基金项目： | 国家"973"重点基础研究发展规划项目(2008CB418006);国家"水体污染控制与治理"科技重大专项(2009ZX07106-001) |
| 摘 要： | 使用Agilent1260型高效液相色谱仪,建立定量测定天然水体中5种微囊藻毒素（MC-RR、YR、LR、LA、LY）的分析方法。比较甲醇/三氟乙酸（TFA）水溶液与甲醇/磷酸两种流动相体系,待测物质的响应程度,结果发现,TFA作为缓冲溶液时,基线较磷酸稳定,因此,选择甲醇/TFA水溶液作为流动相。比较不同TFA体积比例,待测物质的分离程度,结果显示,甲醇/0.02%的TFA水溶液作为流动相时,5种MCs含量的分离度较好。比较等度和梯度洗脱条件,发现,梯度洗脱条件5种MCs的分离度较等度洗脱条件好。该方法 MC-RR、YR、LR、LA、LY的最低检出限（LOD）分别为0.015、0.035、0.018、0.032和0.026μg/L,最低定量限（LOQ）分别是0.049、0.117、0.062、0.106和0.086μg/L。在0.10～1.50μg/mL的线性范围内,相关系数R2≥0.99,实际水样回收率为73.9%～103.3%。 |
| 关 键 词： | 微囊藻毒素-RR YR LR LA LY 高效液相色谱 天然水体 |
| 收稿时间： | 9/1/2011 12:00:00 AM |
Detection of 5 variants microcystins in waters using high performance liquid chromatography |
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| Mao Jingying,Yang Min,Di Yian,Cao Jinling,Ren Lijun,Xu Qigong and Xi Beidou.Detection of 5 variants microcystins in waters using high performance liquid chromatography[J].Techniques and Equipment for Environmental Pollution Control,2012,6(11):3882-3888. | |
| Authors: | Mao Jingying Yang Min Di Yian Cao Jinling Ren Lijun Xu Qigong and Xi Beidou |
| Institution: | 1. School of Earth Science and Environmental Engineering, Southwest Jiaotong University, Chengdu 610031, China;2. Sino-Japanese Friendship Environment Protection Center,Beijing 100029;3. Laboratory of Water Environmental System Engineering, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;1. School of Earth Science and Environmental Engineering, Southwest Jiaotong University, Chengdu 610031, China;2. Sino-Japanese Friendship Environment Protection Center,Beijing 100029;3. Laboratory of Water Environmental System Engineering, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;2. Sino-Japanese Friendship Environment Protection Center,Beijing 100029;3. Laboratory of Water Environmental System Engineering, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;4. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;3. Laboratory of Water Environmental System Engineering, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;4. State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China |
| Abstract: | |
| Keywords: | microcystin-RR YR LR LA LY high performance liquid chromatography(HPLC) natural water bodies |
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