土霉素生产废水生物处理装置中微生物群落结构特征解析

抗生素对细菌具有强抑制作用,从而会影响废水生物处理系统中的微生物群落结构。在用定量PCR方法对土霉素生产废水处理装置(进水中土霉素浓度为1 662.1±248.6μg/L)中的细菌16S rRNA基因和真菌18S rRNA基因的含量进行比较的基础上,利用16S rRNA基因克隆文库方法对污泥中的细菌群落结构进行了详细解析。定量PCR结果显示,土霉素废水活性污泥中真菌(18S rRNA)/细菌(16S rRNA)基因的拷贝数比例高达1.2,明显较高于非抗生素肌苷废水活性污泥中的比例1.52×10-6,表明真菌对于土霉素废水中有机物的去除可能发挥重要作用。细菌克隆文库分析结果显示,Alpha变形菌和Beta变形菌是主要的优势菌,比例分别为23.7%和22.0%,其次是酸杆菌(17.0%)和拟杆菌(11.9%)。

Microbial community structures analysis in a biological plant treating oxytetracycline production wastewater

As antibiotics can cause strong inhibition of bacteria,their presence will influence microbial community structure in wastewater treatment plants.The abundances of bacterial and fungal populations in an oxytetracycline-production wastewater(influent oxytetracycline 1 662.1±248.6 μg/L) were measured by quantitative real-time PCR,and bacterial community structure was examined by 16S rDNA clone library construction.The ratio of fungi/bacteria valued 1.2 in oxytetracycline-production wastewater,much higher than that(1.52×10-6) in inosine-production wastewater with no antibiotic.The results indicate that fungi may play an important role in COD removal of oxytetracycline-production wastewater treatment.Bacterial community structure analysis shows that Alphaproteobacteria(23.7%) and Betaproteobacteria(22.0%) are predominant groups,and then Acidobacteria(17.0%) and Bacteroidetes(11.9%).