| 不同状态下的同一污泥胞外聚合物提取方法研究 |
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| 引用本文： | 邹小玲,许柯,丁丽丽,任洪强.不同状态下的同一污泥胞外聚合物提取方法研究[J].环境工程学报,2010,4(2):436-440. |
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| 作者姓名： | 邹小玲 许柯 丁丽丽 任洪强 |
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| 作者单位： | 南京大学环境学院污染控制与资源化研究国家重点实验室,南京,210093 |
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| 基金项目： | 江苏省社会发展项目（BS2006049） |
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| 摘 要： | 以由厌氧污泥向好氧污泥转变前后2种不同状态下的污泥为研究对象,采用5种不同方法对污泥胞外聚合物(EPS)进行了提取实验。实验结果表明,NaOH法提取的EPS含量最高,但会导致胞内物质泄漏。CER和离心提取法较温和,但EPS产量不高。甲醛-NaOH和H2SO4法提取的EPS含量较高,且无大量细胞自溶发生,是有效的EPS提取方法。厌氧污泥转变为好氧污泥后蛋白质/多糖(PN/PS)比例有所增加,表明EPS各组分含量发生改变。红外分析表明厌氧污泥转变为好氧污泥前后EPS中主要组成成分没有发生改变,但各成分含量有所增加,特征明显的峰表明EPS中存在蛋白质、多聚糖。
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| 关 键 词： | 厌氧污泥 好氧污泥 胞外聚合物 提取 |
Study on extraction of extracellular polymeric substances from same sludge under different conditions |
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| Zou Xiaoling,Xu Ke,Ding Lili and Ren Hongqiang.Study on extraction of extracellular polymeric substances from same sludge under different conditions[J].Techniques and Equipment for Environmental Pollution Control,2010,4(2):436-440. |
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| Authors: | Zou Xiaoling Xu Ke Ding Lili and Ren Hongqiang |
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| Institution: | State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210093, China,State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210093, China,State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210093, China and State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing 210093, China |
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| Abstract: | Five different extracellular polymeric substances extraction methods were studied on the same sludge under anaerobic and aerobic conditions.Results showed that NaOH extraction gave the greatest EPS yield,while it released intracellular substances.Cation exchange resin(CER) and centrifugation extraction were gentle methods,while they gave low EPS yield.Formaldehyde-NaOH and sulfuric acid extraction gave higher EPS yield and less extensive cellular disruption,therefore they were most effective in extracting E... |
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| Keywords: | anaerobic sludge aerobic sludge extracellular polymeric substances extraction |
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