| 呼吸法测定硝化菌产率系数实验研究 |
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| 引用本文： | 任宏洋,张代钧,丛丽影,张萍.呼吸法测定硝化菌产率系数实验研究[J].环境工程学报,2010,4(8):1726-1728. |
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| 作者姓名： | 任宏洋 张代钧 丛丽影 张萍 |
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| 作者单位： | 1.西南石油大学化学化工学院，成都 610500;2.重庆大学资源及环境科学学院，西南资源开发及环境灾害控制工程教育部重点实验室，重庆 400030,2.重庆大学资源及环境科学学院，西南资源开发及环境灾害控制工程教育部重点实验室，重庆 400030,2.重庆大学资源及环境科学学院，西南资源开发及环境灾害控制工程教育部重点实验室，重庆 400030,2.重庆大学资源及环境科学学院，西南资源开发及环境灾害控制工程教育部重点实验室，重庆 400030 |
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| 基金项目： | 国家自然科学基金资助项目（50378094）；西南石油大学校级科技基金项目 |
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| 摘 要： | 基于硝化过程中合成代谢和能量代谢耦合原理,分析硝化过程中的电子流,建立自养硝化菌细胞生长与氨氮氧化耗氧量之间的关系。采用间歇实验方法,通过电解质呼吸仪测定硝化过程的累积耗氧量和耗氧速率,使用M ann-Ken-dell趋势检验方法确定实验中硝化菌进入内源呼吸的时刻以及内源呼吸过程中的耗氧速率,估算出氨氮氧化的耗氧量。计算结果表明本实验中硝化菌产率系数为0.2345±0.0115 mg COD/mg NH4+-N。
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| 关 键 词： | 呼吸测量 硝化菌 产率系数 |
Experimental study on determination of nitrifying bacteria yield coefficients using respirometry |
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| Ren Hongyang,Zhang Daijun,Cong Liying and Zhang Ping.Experimental study on determination of nitrifying bacteria yield coefficients using respirometry[J].Techniques and Equipment for Environmental Pollution Control,2010,4(8):1726-1728. |
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| Authors: | Ren Hongyang Zhang Daijun Cong Liying and Zhang Ping |
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| Institution: | 1.School of Chemistry and Chemical Engineering, Southwest Petroleum University, Chengdu 610500,China;2.Key Laboratory for Exploitation of Southwest China Resources & Environmental Disaster Control Engineering,Ministry of Education, College of Resources an,2.Key Laboratory for Exploitation of Southwest China Resources & Environmental Disaster Control Engineering,Ministry of Education, College of Resources and Environmental Science, Chongqing University, Chongqing 400030, China,2.Key Laboratory for Exploitation of Southwest China Resources & Environmental Disaster Control Engineering,Ministry of Education, College of Resources and Environmental Science, Chongqing University, Chongqing 400030, China and 2.Key Laboratory for Exploitation of Southwest China Resources & Environmental Disaster Control Engineering,Ministry of Education, College of Resources and Environmental Science, Chongqing University, Chongqing 400030, China |
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| Abstract: | Based on the coupling of anabolism and energy metabolism in nitrification, the electron stream in nitrifying process was analyzed, and relationship between autotrophic nitrifying bacteria growth and oxygen consuming in ammonium oxidizing process was established. Accumulated oxygen consuming and oxygen uptake rate (OUR) in nitrifying process were measured by electrolytic respirometer in batch experiments. With the method of Mann Kendell trend test, the time nitrifying bacteria got in endogenous respiration and the OUR in endogenous respiration were determined, and then the oxygen consuming in ammonium oxidizing was calculated. Nitrifying bacteria yield coefficients in experiments was estimated, which was 0.2345±0.0115 mg COD/mg NH+4-N. |
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| Keywords: | respirometry nitrifying bacteria yield coefficients |
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