固定化降解菌Q5去除喹啉的性能研究

为了研究固定化微生物在土壤生物修复中的应用,以实验室筛选出来的高效降解菌 Q5 为生物活性物质,利用生物大分子仿生合成出的纳米多孔氧化硅为载体,通过表面吸附同定化方法将其固定,制备出固定化微生物.考察固定化微牛物初始 pH 值、温度、摇床转速和菌种的接种量对喹啉去除的影响,得到适宜的去除条件,在相同条件下比较固定化微生物与游离菌种对底物的去除情况,研究单一固定化菌种对不同浓度的喹啉的去除情况,考察固定化微生物的稳定性.实验结果表明,菌株 Q5 经固定化后,对喹啉的去除能力大大增强,在 500 mg/L 浓度下,40 h 固定化 Q5 对底物去除率达96.6%,远高于未固定化 Q5 的去除率 56.1%;对于高底物浓度,固定化微生物的去除效果明显,初始底物浓度为1 500 mg/L,反应 70 h 后去除率为 91.6%,且这种固定化微生物的重复使用性能良好.

The capability of removing quinoline of immobilized bacteria Q5

To study the application of immobilized microorganisms in bioremediation of soil, screened high efficiency degrading bacteria Q5 as bioactive substances and nanoporous silica synthesized by bio macromolecule bionic technology as a carrier, immobilized microorganisms were obtained by surface adsorption. The optimum degrading condition was found by evaluating the influences of the initial pH, temperature, shaking rate and inoculation amount. The degrading effect of immobilized microorganisms was compared with that of suspended bacteria under the same condition. The quinoline degradation by single immobilized bacteria with different quinoline concentrations was studied and the stability of immobilized microorganisms was also observed. It was shown that the degrading ability of strain Q5 was enhanced drastically after being immobilized. The degrading rate to quinoline of immobilized Q5 after 40 h reaction was 96.6% in 500 mg/L quinoline concentration, higher than that of the strain Q5 without immobilization (56.1%). The degrading effect was also perfect (91.6% after 70 h reaction) in a high substrate concentration (1 500 mg/L), and this kind of immobilized microorganisms was of good reusing property.