| 产漆酶食用菌的粗酶液对多环芳烃降解研究 |
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| 引用本文： | 李烜桢,林先贵,尹睿,张晶,胡君利,安琼,李晶,陈虹.产漆酶食用菌的粗酶液对多环芳烃降解研究[J].环境工程学报,2010,4(12):2888-2892. |
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| 作者姓名： | 李烜桢 林先贵 尹睿 张晶 胡君利 安琼 李晶 陈虹 |
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| 作者单位： | 1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008；2.中国科学院南京土壤研究所香港浸会大学土壤与环境联合开放实验室, 南京 210008；3.中国科学院研究生院, 北京 100049,1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008；2.中国科学院南京土壤研究所香港浸会大学土壤与环境联合开放实验室, 南京 210008,1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008；2.中国科学院南京土壤研究所香港浸会大学土壤与环境联合开放实验室, 南京 210008,1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008；2.中国科学院南京土壤研究所香港浸会大学土壤与环境联合开放实验室, 南京 210008,1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008；2.中国科学院南京土壤研究所香港浸会大学土壤与环境联合开放实验室, 南京 210008,1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008；2.中国科学院南京土壤研究所香港浸会大学土壤与环境联合开放实验室, 南京 210008,4.南京中科院跨克科技有限责任公司, 南京 210008,1.中国科学院南京土壤研究所土壤环境与污染修复重点实验室, 南京 210008 |
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| 基金项目： | 国家高技术研究发展计划（863）重点项目（2007AA061101）;国家自然科学基金资助项目（40801091） |
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| 摘 要： | 漆酶是一种含铜的多酚氧化酶,可以降解多种有机污染物,其中包括多环芳烃。但是由于纯漆酶的价格昂贵,限制了在环境修复中的应用。采用液态和固态2种方式培养了食用菌Pleurotus ostreatus和Coprinus comatus,并考察了固态培养的粗酶液对多环芳烃的降解和去毒能力,结果发现2种真菌均可产漆酶,但不产木质素过氧化物酶和锰过氧化物酶,其中固态培养的漆酶产量高于液态培养,且C.comatus的漆酶产量大于P.ostreatus。2种真菌固态培养的粗酶液对多环芳烃均有一定的降解和去毒作用,且P.ostreatus粗酶液的漆酶活性虽然较低,但是对多环芳烃降解率和去毒效果却优于C.comatus,这可能是P.ostreatus的粗酶液中含有较多的调节物质造成的。P.ostreatus的固态发酵粗酶液对蒽、苯并a]芘、苯并a]蒽、苊等均有较高的降解率(50%以上),但是对生物有效性高的萘的降解率却较低,这种降解特征可能与底物的电离势大小有关。
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| 关 键 词： | 食用菌 粗酶液 多环芳烃 降解 |
| 修稿时间： | 1/5/2010 12:00:00 AM |
Study on degradation of polycyclic aromatic hydrocarbons by crude enzymes from laccase-produced mushrooms |
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| Li Xuanzhen,Lin Xiangui,Yin Rui,Zhang Jing,Hu Junli,An Qiong,Li Jing and Chen Hong.Study on degradation of polycyclic aromatic hydrocarbons by crude enzymes from laccase-produced mushrooms[J].Techniques and Equipment for Environmental Pollution Control,2010,4(12):2888-2892. |
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| Authors: | Li Xuanzhen Lin Xiangui Yin Rui Zhang Jing Hu Junli An Qiong Li Jing and Chen Hong |
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| Institution: | 1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science, Chinese Academy of Sciences and Hongkong Baptist University, Nanjing 210008, China; 3. Graduate University of Chinese Academy of Sciences, Beijing 100049, China,1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science, Chinese Academy of Sciences and Hongkong Baptist University, Nanjing 210008, China,1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science, Chinese Academy of Sciences and Hongkong Baptist University, Nanjing 210008, China,1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science, Chinese Academy of Sciences and Hongkong Baptist University, Nanjing 210008, China,1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science, Chinese Academy of Sciences and Hongkong Baptist University, Nanjing 210008, China,1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; 2. Joint Open Laboratory of Soil and the Environment, Institute of Soil Science, Chinese Academy of Sciences and Hongkong Baptist University, Nanjing 210008, China,4. Kuake Science and Technology Limited Liability Company, Chinese Academy of Sciences, Nanjing 210008, China and 1. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China |
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| Abstract: | |
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| Keywords: | mushroom crude enzymes polycyclic aromatic hydrocarbons degradation |
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