丛毛单胞菌对邻甲酚及对甲酚的降解特性

考察不同底物浓度、pH及金属离子对丛毛单胞菌(Comamonas sp.)Z1(以下简写为Z1)降解邻甲酚与对甲酚的影响,并利用高效液相色谱/质谱对降解产物进行分析。结果表明:(1)Z1可利用邻甲酚与对甲酚作为唯一碳源进行生长,36h内能将25~75mg/L邻甲酚、25~200mg/L对甲酚完全降解。(2)Z1可在pH为8.0时将50mg/L邻甲酚或100mg/L对甲酚快速降解。(3)Mg2+对Z1生长、底物降解产生促进作用,Co2+、Ni2+、Cu2+等金属离子则具有抑制作用。(4)推断Z1能利用龙胆酸与儿茶酚途径降解邻甲酚,通过龙胆酸途径降解对甲酚。

Degradation characteristics of o-cresol and p-cresol by Comamonas sp .

The effects of different conditions on biodegradation of o-cresol and p-cresol by Comamonas sp.Z1(hereinafter to be referred as Z1)were investigated,including the initial concentration,pH and metal ions.The degradation products were also analyzed by high performance liquid chromatography-mass spectrometry.The results showed:(1)Z1 could utilize o-cresol and p-cresol as the sole carbon source for growth and completely degrade 25-75 mg/L o-cresol and 25-200 mg/L p-cresol within 36 h.(2)Z1 could quickly degraded 50 mg/L o-cresol or 100 mg/L p-cresol at pH 8.0.(3)Mg2+ could accelerate the biodegradation and growth of Z1,while other metal ions like Co2+,Ni2+ and Cu2+ showed certain inhibitory influences.(4)It was inferred that Z1 could use gentisic acid and catechol pathways to degrade o-cresol,the gentisic acid pathway to degrade p-cresol.