| 一株亚硝酸盐降解菌的分离鉴定与系统发育分析 |
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| 引用本文： | 王会聪,曹海鹏,何珊,杨先乐.一株亚硝酸盐降解菌的分离鉴定与系统发育分析[J].环境污染与防治,2012,34(4):32-36. |
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| 作者姓名： | 王会聪 曹海鹏 何珊 杨先乐 |
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| 作者单位： | 1. 上海海洋大学国家水生动物病原库,上海,201306
2. 上海师范大学教育系,上海,200234 |
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| 基金项目： | 国家“863计划”项目，国家自然科学基金资助项目，公益性行业(农业)科研专项，现代农业产业技术体系建设专项 |
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| 摘 要： | 从水产养殖污泥中分离筛选了一株优良的亚硝酸盐降解菌AQ-3,其在1.0×107 cfu/mL时对50mg/L亚硝酸盐的去除率高达99.47%。通过细菌自动鉴定系统(API ID32GN系统)以及16SrDNA系统发育分析,菌株AQ-3被鉴定为鲍曼氏不动杆菌(Acinetobacter baumannii)(JF751054),其16SrDNA序列与基因库中不动杆菌属菌株的16SrDNA序列有99%的同源性,而且与鲍曼氏不动杆菌菌株14(FJ907197)的亲缘关系最近。此外,菌株AQ-3同时含有nirS基因、nirK基因和norB基因,这些基因对AQ-3高效降解亚硝酸盐具有重要的意义。
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| 关 键 词： | 亚硝酸盐 鲍曼氏不动杆菌 鉴定 系统发育分析 |
Isolation, identification and phylogenetic analysis of a nitrite degrading bacterium |
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| WANG Huicong
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CAO Haipeng
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HE Shan
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YANG Xianle.Isolation, identification and phylogenetic analysis of a nitrite degrading bacterium[J].Environmental Pollution & Control,2012,34(4):32-36. |
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| Authors: | WANG Huicong CAO Haipeng HE Shan YANG Xianle |
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| Institution: | 1.(1.National Collection Center for Aquatic Pathogens,Shanghai Ocean University,Shanghai 201306;2.Education Department,Shanghai Normal University,Shanghai 200234) |
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| Abstract: | A potential nitrite degrading bacterium AQ-3 was isolated and screened from the aquaculture sediment.The nitrite removal rate of AQ-3 was detected as 99.47%.Strain AQ-3 was identified as Acinetobacter baumannii(JF751054) through API ID32GN bacterial identification system and 16S rDNA sequence analysis.Its 16S rDNA sequence had homology of 99% with those of Acinetobacter sp.submitted to GenBank,and showed the closest relative to Acinetobacter baumannii strain 14(FJ907197).In addition,nirS gene、nirK gene and norB gene all existed in strain AQ-3.This study proved strain AQ-3 was a potential nitrite degrading bacterium,and enriched the microbial resources of nitrite degrading bacterium. |
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| Keywords: | nitrite Acinetobacter baumannii identification phylogenetic analysis |
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