强化土著微生物处理硝基苯和苯胺污染地下水

以硝基苯、苯胺为主要污染物的污染地下水为研究对象,加入激活剂(乳糖、Na2HPO4、乳糖+Na2HPO4、乙醇、牛肉膏、蛋白胨)激活土著微生物,并考察其对土著微生物生长及硝基苯、苯胺降解效果的影响。加入激活剂3d后测各个水样的脱氢酶活性,对培养9d后的水样进行气相色谱/质谱(GC/MS)分析。结果表明,加入乳糖的水样中,其微生物相对增长率达157.2%,硝基苯、苯胺的相对去除率分别为14.90%和0.79%;加入Na2HPO4和乙醇的水样中,其微生物增长和硝基苯、苯胺降解情况均没有明显变化;加入乳糖+Na2HPO4的水样中,微生物相对增长率达180.3%,硝基苯、苯胺的相对去除率分别为24.20%和1.21%;加入牛肉膏的水样中,微生物的相对增长率为830.7%,硝基苯、苯胺的相对去除率分别为99.99%和99.67%;加入蛋白胨的水样中,其微生物相对增长率为686.0%,硝基苯、苯胺的相对去除率分别为99.33%和58.94%。GC/MS分析结果表明,加入激活剂后对氯苯胺、1-甲基-4-硝基苯等其他有机物的降解率均有提高。由此可见,通过激活土著微生物修复有机物污染地下水是可行的。

Treatment of the nitrobenzene and aniline-contaminated groundwater by activated indigenous microorganism

The nitrobenzene and aniline-contaminated groundwater was selected as the object to study the performance of activated indigenous microorganism for nitrobenzene and aniline degradation.In this paper,different activators(lactose;Na2HPO4;Na2HPO4+lactose;ethanol;beef extract;peptone) were added to contaminated groundwater to active the indigenous microorganisms,the dehydrogenase activity of water sample was measured at the 3th d and water samples was analyzed by full scan GC/MS at the 9th d.The results indicated that the relative growth rate of microorganisms was up to 157.2% after adding lactose in water sample and the degradation rate of nitrobenzene and aniline increased by 14.90% and 0.79% respectively;Na2HPO4 and ethanol had little activation effect on indigenous microorganism,the growth rate of microorganisms and the pollutant degradation rate presented no obvious changes.Adding Na2HPO4+lactose in water sample,relative growth rate of microorganisms was 180.3% and the degradation rate nitrobenzene and aniline elevated 24.20% and 1.21% respectively;adding beef extract in water sample,the relative growth rate of microorganisms was 830.7% and the degradation rate of nitrobenzene and aniline elevated 99.99% and 99.67% respectively;adding peptone in water sample,the relative growth rate of microorganisms was 686.0% and the degradation rate of nitrobenzene and aniline elevated 99.33% and 58.94% respectively.The full scan GC/MS spectra showed that the degradation of chloroaniline,1-methyl-4-nitrobenzene and other organic matter was enhanced after adding different activators,so it was feasible to remediate the organic contamination groundwater by activating the indigenous microbial.