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| 垃圾堆肥高效纤维素分解菌的筛选与培育技术 | |
| 引用本文： | 席北斗,孟伟,刘鸿亮,王琪,黄国和,曾光明.垃圾堆肥高效纤维素分解菌的筛选与培育技术[J].环境污染与防治,2002,24(6):339-341. |
| 作者姓名： | 席北斗 孟伟 刘鸿亮 王琪 黄国和 曾光明 |
| 作者单位： | 1. 中国环境科学研究院,北京,100012;EVSE Faculty of Engineering University of Regina 2. 中国环境科学研究院,北京,100012 3. 湖南大学环境科学与工程系,长沙,410082 |
| 摘 要： | 从堆肥、马粪、果园土、污泥等20个样品中，分离筛选出3株对滤纸分解旺盛的纤维素分解菌：C1、C2、C3，并外购康氏木霉、白腐菌、变色栓菌一起作为出发株，经紫外诱变处理后，在含葡萄糖的产酶培养基平板上筛选到能形成较大透明圈的突变株，并进行CMC酶活、微晶纤维素酶活及天然粗纤维分解能力测定。实验结果证明白腐菌经紫外线照射60s诱变而得的C16不仅透明圈大，CMC酶活高（60.08U/mL）是出发株的2倍，而且其对天然粗纤维分解能力强，10d分解率达35％。 |
| 关 键 词： | 垃圾堆肥 筛选 培育技术 纤维素分解菌 康氏木霉 白腐菌 变色栓菌 紫外诱变 垃圾处理 |
| 修稿时间： | 2002年4月18日 |
Study on preparation technology of cellulolytic organisms for composting |
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| Xi Beidou,Meng Wei,Liu Hongliang,et al..Study on preparation technology of cellulolytic organisms for composting[J].Environmental Pollution & Control,2002,24(6):339-341. | |
| Authors: | Xi Beidou Meng Wei Liu Hongliang |
| Institution: | Xi Beidou,Meng Wei,Liu Hongliang,et al. Chinese Research Academy of Environmental Science,Beijing 100084 |
| Abstract: | Separated three strains of cellulose from composting,ordure of horse, soil of gardern and sewage sludge, together with Trichoderma koningii, White rot, Trametes versicolor as original strains. The strains continually mutagenized by ultraviolet radiation, resistant mutants were selected on the producing enzyme medium plate in the presence of glucose. These mutants can shape bigger transparent ring. C - {16 } mutagenized from White-rot mutant can increase 2 times(60.08 U/mL), and the rate of hydrolyzed natural cellulose is about 35% for 10 days. |
| Keywords: | Cellulolytic organisms Trichoderma koningii White rot Trametes versicolor Mutagenized by ultraviolet radiation |
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