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Study on biodegradation process of lignin by FTIR and DSC |
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| Authors: | Yang Liu Tianjue Hu Zhengping Wu Guangming Zeng Danlian Huang Ying Shen Xiaoxiao He Mingyong Lai Yibin He |
| Institution: | 1. College of Environmental Science and Engineering, Hunan University, Changsha, 410082, People’s Republic of China 2. Key Laboratory of Environmental Biology and Pollution Control, Ministry of Education, Hunan University, Changsha, 410082, People’s Republic of China 3. School of Chemistry and Chemical Engineering, Central South University, Changsha, 410083, People’s Republic of China |
| Abstract: | The biodegradation process of lignin by Penicillium simplicissimum was studied to reveal the lignin biodegradation mechanisms. The biodegradation products of lignin were detected using Fourier transform infrared spectroscopy (FTIR), UV–Vis spectrophotometer, different scanning calorimeter (DSC), and stereoscopic microscope. The analysis of FTIR spectrum showed the cleavage of various ether linkages (1,365 and 1,110 cm?1), oxidation, and demethylation (2,847 cm?1) by comparing the different peak values in the corresponding curve of each sample. Moreover, the differences (Tm and ΔHm values) between the DSC curves indirectly verified the FTIR analysis of biodegradation process. In addition, the effects of adding hydrogen peroxide (H2O2) to lignin biodegradation process were analyzed, which indicated that H2O2 could accelerate the secretion of the MnP and LiP and improve the enzymes activity. What is more, lignin peroxidase and manganese peroxidase catalyzed the lignin degradation effectively only when H2O2 was presented. |
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