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Development of dioxin toxicity evaluation method in human milk by enzyme-linked immunosorbent assay--assay validation for human milk |
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| Authors: | Sugawara Yukio Saito Koichi Ogawa Masahiko Kobayashi Susumu Shan Guomin Sanborn James R Hammock Bruce D Nakazawa Hiroyuki Matsuki Yasuhiko |
| Institution: | a Cosmo Oil Co. Ltd., Tokyo 105-8528, Japan b Dioxin Research Group, Saitama Institute of Public Health, Saitama 338-0824, Japan c Department of Entomology and the UCD Cancer Center, University of California, Davis, CA 95616, USA d Department of Analytical Chemistry, Hoshi University, Tokyo 142-8501, Japan e Food and Drug Safety Center, Hatano Research Institute, Kanagawa 257-8523, Japan |
| Abstract: | In this study, the development of a toxicity evaluation method for dioxins in human milk by enzyme-linked immunosorbent assay (ELISA) was reported. A total of 17 human milk samples were tested by ELISA and by gas chromatography/mass spectrometry (GC/MS) to assess whether the ELISA performed on samples obtained from primiparas could be considered as reliable enough for identifying a dioxins contamination in human milk. The concept of toxicity equivalent quantity (TEQ) screening was validated by comparing TEQ values for a set of human milk samples to the ELISA responses predicted for those samples. A fairly good correlation (r=0.920) between immunoassay and GC/MS was achieved for human milk. This ELISA should be useful for biological samples monitoring. |
| Keywords: | TCDD Immunoassay Validation PCDDs PCDFs GC/MS |
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