Interference by the activated sludge matrix on the analysis of soluble microbial products in wastewater |
| |
Authors: | Potvin Christopher M Zhou Hongde |
| |
Institution: | University of Guelph, School of Engineering, Guelph Ontario, Canada N1G 2W1 |
| |
Abstract: | The objective of this study was to demonstrate the effects of complex matrix effects caused by chemical materials on the analysis of key soluble microbial products (SMP) including proteins, humics, carbohydrates, and polysaccharides in activated sludge samples. Emphasis was placed on comparison of the commonly used standard curve technique with standard addition (SA), a technique that differs in that the analytical responses are measured for sample solutions spiked with known quantities of analytes. The results showed that using SA provided a great improvement in compensating for SMP recovery and thus improving measurement accuracy by correcting for matrix effects. Analyte recovery was found to be highly dependent on sample dilution, and changed due to extraction techniques, storage conditions and sample composition. Storage of sample extracts by freezing changed SMP concentrations dramatically, as did storage at 4 °C for as little as 1 d. |
| |
Keywords: | Soluble microbial products Extracellular polymeric substances Wastewater Standard addition Water quality Matrix interference |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|