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Measurement of pyrene in the gills of exposed fish using synchronous fluorescence spectroscopy |
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| Authors: | Liu Xianli Jing Jinqiu Li Shengfang Zhang Guodong Zou Tao Xia Xianyou Huang Weidong |
| Institution: | a Hubei Key Laboratory of Mine Environmental Pollution Control & Remediation, Huangshi Institute of Technology, Huangshi 435003, PR China b School of Chemistry and Materials Engineering, Huangshi Institute of Technology, Huangshi 435003, PR China c School of Higher Vocational Technology, Huangshi Institute of Technology, Huangshi 435003, PR China |
| Abstract: | A synchronous fluorescence spectroscopy (SFS) method was developed for determination of pyrene in the gills of exposed fish. The wavelength differences (Δλ) of 50 nm was maintained between excitation and emission wavelengths and it was found to be suitable for the effective determination of pyrene in fish gills; the peak were observed at λex 334.5 nm. Linear relationships between SFS intensity and the concentration of pyrene in n-hexane solution were established. It was demonstrated that the SFS method was effective, simple, and less expensive, providing an attractive alternative for the rapid analysis of pyrene in fish gills. |
| Keywords: | Fluorescence spectroscopy Synchronous-scan Pyrene Fish gills Measurement |
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