Germination and seedling growth of the water cress Rorippa sp. exposed to the chelant [S,S]-EDDS

With the implementation of the new EU environmental framework directives, high tier risk assessments of chemicals will be increasingly needed. For high production chemicals, additional tests will complement the standard battery for aquatic toxicity assessments (daphnids, algae, and fish). In the context of a new chemical notification at the European Union level, we have developed a seed germination and root elongation toxicity test with the freshwater aquatic plant Rorippa nasturtium-aquaticum (water cress) to confirm the low environmental risk of the chelant S,S]-EDDS. A 14 day semi-static growth inhibition test was conducted with daily renewal of the test solution. No concentration related inhibition was found on the basis of any of the criteria investigated, i.e., time and extent of germination, biomass, number of leaves, stalk and root lengths. The no-observed effect concentration was considered to be >or=387 mg SS-EDDS/l. Although germination was selected as an appropriate endpoint to assess the effect of a chelant on an aquatic plant (other endpoints would have been dependant on essential metals that are chelated in standard culture tests), the absence of dose related effects requires further tests with higher exposure concentrations and/or other toxicant(s) to assess the validity of the test as a general tool for aquatic risk assessment.