Detection of transfluthrin and metofluthrin genotoxicity in the ST cross of the Drosophila Wing Spot Test |
| |
Authors: | Rabia Sar?kaya Burcu Koçak Memmi |
| |
Institution: | 1. Department of Primary School Education, Gazi University, Teknikokullar, 06500 Ankara, Turkey;2. Insecticide Test Laboratory, Hacettepe University, 06800 Beytepe, Ankara, Turkey |
| |
Abstract: | In this study, different concentrations of transfluthrin and metofluthrin have been assayed for genotoxicity by using the Wing Spot Test on Drosophila melanogaster. Standard cross was used in the experiment. Third-instar larvae that were trans-heterozygous for the two genetic markers mwh and flr3 were treated at different concentrations (0.0103 mg mL−1, 0.103 mg mL−1 for transfluthrin and 6 μg mL−1, 60 μg mL−1 for metofluthrin) of the test compounds. Feeding ended with pupation of the surviving larvae and the genetic changes induced in somatic cells of the wing’s imaginal discs lead to the formation of mutant clones on the wing blade. Results indicated that two experimental concentrations of transfluthrin and 60 μg mL−1 metofluthrin showed mutagenic and recombinogenic effects in both the marker-heterozygous (MH) flies and the balancer-heterozygous (BH) flies. |
| |
Keywords: | flr3 flare-3 (Drosophila strain) m multiplication factor mwh multiple wing hairs (Drosophila strain) c Frequency of clone formation per 105 cells LOH Loss of Heterozygosity SMART Somatic Mutation and Recombination Test ST cross Standard cross MH Marker-heterozygous flies (mwh +/+ flare-3) with phenotypically wild-type wings BH Balancer-heterozygous flies (mwh +/+ TM3 Bds) with phenotypically serrate wings |
本文献已被 ScienceDirect 等数据库收录! |