连续培养酱油曲霉（Aspergillussojae）产微生物絮凝剂的研究

为了利用廉价材料规模化生产微生物絮凝剂，以味精废水作为廉价培养基质，对酱油曲霉的摇瓶连续培养和发酵罐连续培养进行了研究。摇瓶中以5％的接种量进行连续培养，最适温度在30～33℃之间，每6h替换一次新鲜废水培养基，5次替换新鲜废水培养基后最大絮凝率仍达到97．8％。在发酵罐扩大连续培养中以5％的接种量接种后经过9h预培养达到稳定生产絮凝剂后，以4mL／min的补料流量进行连续培养，生产的絮凝剂产量达到2．392g／L，且最大絮凝率为98．1％。生产36h后对发酵系统中菌体进行稀释，使菌体量保持在50—200g／L之间可持续进行生产。

Research on producing bioflocculant through continuous cultivation of Aspergillus sojae

In order to achieve scaled-up production of bioflocculant,the characteristics of continuous culti- vation of AspergiUus sojae in shake flasks and fermenter using glutamic acid wastewater as cheap culture material were studied. The experimental results showed that with 5% inoculum dosage,the maximal flocculation rate remained 97.8% in the shake flasks when replacing the fresh wastewater every 6 h 5 times at the optimal temperature of 30 -33℃. In the fermenter, the continuous cultivation was conducted with continuous feeding after the production process of bioflocculant was stabilized with preculture for 9 h at inoculum dosage of 5%. The bioflocculant yield was 2. 392 g/L and the maximal flocculation rate reached 98.1% when the feed flow rate was 4 mL/ min. After running for 36 h,the strains in the fermenter were diluted to the range of 50 - 200 g/L for the succedent continuance production.