蒽降解菌T2的最佳培养条件及其对蒽的生物降解动力学研究

从长期被石油污染的土壤中筛选得到一株以蒽为惟一碳源的混合菌T2，在接种量为1％，pH为7，温度为30℃，摇床转速为120r／min，蒽的初始浓度为100mg／L的条件下培养5d后，其对蒽的降解率可以达到56．6％。通过单因素实验和正交实验对菌种T2的培养条件进行研究，得到菌种T2的最佳培养条件为：接种量为5％，pH为6，温度为35℃，蒽的初始浓度为40mg／L时，最适合菌种生长。另外，菌种T2对蒽的降解动力学实验的结果表明，蒽的残留浓度Y（mg／L）与时间t（h）符合方程y=2．544e（-0.00275）t.

Study on optimal cultivating bacteria and its biological conditions of anthracene degrading degrading kinetics to anthracene

A mixed bacteria T2 which used anthracene as the sole carbon source was screened from the long-term petroleum polluted soil. When the inoculation amount of T2, pH, temperature, shaking-bed＇s speed and the initial concentration of anthracene were 1% , 7, 30℃, 120 r/rain and 100 rag/L, respectively, the degrading rate of T2 to anthracene could reach 56.6% through 5 days＇ cultivation. The optimal cultivating conditions of strain T2 were obtained through studying the cultivating conditions of T2 , the result showed that when the inoculation amount of T2, pH, temperature and the initial concentration of anthracene were 5% , 6, 35℃ and 40 mg/L, respectively, the strain grew best. In addition, the degrading kinetics experiment of strain T2 to anthracene was carried out, the result showed that the residual concentration of anthracene Y（mg/L） and time t （h） was in line with the equation Y=2. 544 e（ -0.00275）t.