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甲醛致核酸损伤作用的实验研究
引用本文:袭著革,晁福寰,杨丹凤,孙咏梅,李官贤,张华山,张伟,杨玉花,刘焕亮.甲醛致核酸损伤作用的实验研究[J].环境科学学报,2004,24(4):719-722.
作者姓名:袭著革  晁福寰  杨丹凤  孙咏梅  李官贤  张华山  张伟  杨玉花  刘焕亮
作者单位:军事医学科学院卫生学环境医学研究所,天津,300050
基金项目:国家科技部"十五"攻关项目(2001BA704B01),国家科技部社会公益基金(2001DIA1001)
摘    要:应用单细胞凝胶电泳技术(SCGE)和液相色谱-电化学检测法(HPLC-EC)研究了典型环境污染物甲醛对DNA分子的损伤效应.结果表明:甲醛不仅可诱导人外周血淋巴细胞DNA发生链断裂,还可引起DNA-DNA,DNA-蛋白质交联;甲醛与小牛胸腺DNA的体外作用较弱,但在铁离子介导下对DNA的氧化能力增强,可产生一定量的8-羟基脱氧鸟苷(8-OHdG)加合物:动物实验表明甲醛诱导大鼠肺组织DNA氧化损伤生成8-OHdG,提示甲醛较强的遗传毒性.

关 键 词:甲醛  核酸损伤  DNA链断裂  DNADNA交联  DNA蛋白质交联  8羟基脱氧鸟苷
文章编号:0253-2468(2004)04-0719-04
收稿时间:8/7/2003 12:00:00 AM
修稿时间:2003年8月7日

Experimental study of the DNA damage induced by formaldehyde
XI Zhuge,CHAO Fuhuan,YANG Danfeng,SUN Yongmei,LI Guanxian,ZHAN Huashan,ZHAN Wei,YANG Yuhua and LIU Huanliang.Experimental study of the DNA damage induced by formaldehyde[J].Acta Scientiae Circumstantiae,2004,24(4):719-722.
Authors:XI Zhuge  CHAO Fuhuan  YANG Danfeng  SUN Yongmei  LI Guanxian  ZHAN Huashan  ZHAN Wei  YANG Yuhua and LIU Huanliang
Institution:Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050,Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050 and Institute of Health and Environmental Medicine, Academy of Military Medical Sciences, Tianjin 300050
Abstract:The DNA damages induced by the typical environmental pollutant formaldehyde was studied with single cell gel electrophoresis(SCGE) and high performance liquid chromatography with electrochemical detection(HPLC-EC). The results showed that formaldehyde could not only cause DNA strand breakage but also DNA DNA, DNA protein crosslinks of lymphocytes of human peripheral blood. The reaction of formaldehyde with DNA in vitro is weak, but the oxidative ability is enhanced and the reaction could produce a number of 8-OHdG adducts mediated by the Fe2+. The animal experiment showed that formaldehyde could cause the oxidative DNA damage of rat lung tissues, which suggested that formaldehyde have the stronger genotoxicity.
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