组合介质的微生物富集效果及其去除太湖水源水中微囊藻毒素的研究

采用组合介质进行改善太湖水源地水质的中试研究.应用定量PCR分析发现组合介质上富集了大量具有溶藻能力的假单胞菌和芽孢杆菌,表明组合介质对太湖土著溶藻细菌具有良好的富集效果.微生物的空间分布解析结果表明,细菌总数、硝化细菌及溶藻细菌(假单胞菌、芽孢杆菌)的分布具有显著特征,在反应装置纵向的中段、出水端及垂直方向的中层,组合介质上的溶藻细菌等特定微生物在密度及活性上处于相对优势,这与微生物的功能、生长所需的微环境及基质条件密切相关.组合介质通过富集微生物改善了太湖梅梁湾水源地的水质,对总藻毒素TMC、TMC-RR、TMC-LR、胞外藻毒外EMC、Chl-a的平均去除率为64.8%、72.7%、62.9%、55.4%、30.1%.

Study on microbe enrichment effect of assembled-medium and its removal to microcystins in Lake Taihu water source

Pilot study was conducted to improve water source quality in Lake Taihu by an assembled medium. By using Polymerase Chain Reaction(PCR) quantificational analysis large numbers of Pseudomonas sp. and Bacillus sp. with the ability of lysing algae were found to enrich on assembled medium, which indicated the assembled medium could have an effect on the enrichment of the indigenous lysing-algae bacteria in Lake Taihu. The analytical result of microbe's spatial distribution demonstrated that distributions of the total bacteria number, nitrobacteria and lysing-algae bacteria including Pseudomonas sp. and Bacillus sp. were of distinct characteristic, which was closely related to microbe's function, growth microhabitat and substrate condition. For instance, lysing-algae bacteria were comparatively predominant in the middle section, outlet part and middle layer of the assembled medium. The removal rates of TMC, TMC-RR, TMC-LR, EMC and Chl-a after water passing through the assembled medium were averagely at 64.8%, 72.7%, 62.9%, 55.4%, and 30.1% respectively. It was obvious the assembled medium enriched microbe could improve source water quality of Meiliangwan Bay of Lake Taihu.