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甲醛吸入致小鼠蛋白质氧化损伤作用的研究
引用本文:段丽菊,朱燕,胡青莲,刘英帅,杨旭.甲醛吸入致小鼠蛋白质氧化损伤作用的研究[J].环境科学学报,2005,25(6):851-854.
作者姓名:段丽菊  朱燕  胡青莲  刘英帅  杨旭
作者单位:华中师范大学生命科学学院环境科学实验室,武汉,430079
基金项目:国家“十五”科技攻关课题 (No .2 0 0 1BA70 4B0 1)~~
摘    要:为了探讨在气态甲醛中暴露后小鼠的脑、心和肝组织蛋白质的氧化损伤程度及其发生机制,用不同剂量的气态甲醛对小鼠进行连续动态染毒处理72h ,用2 ,4-二硝基苯肼比色法测定小鼠脑、心和肝组织蛋白质的羰基含量,用以判断蛋白质的氧化损伤程度.结果显示:吸入0. 68mg·m- 3甲醛后,小鼠的脑、心和肝蛋白质的羰基含量下降(P <0 0 1,P <0 . 0 1,P <0. 0 5 ) ;吸入3 0mg·m- 3甲醛后,小鼠的心和肝的蛋白质羰基含量均显著升高(P <0 . 0 1) ,而脑的蛋白质羰基含量与对照组相比无显著差异(P >0 . 0 5 ) .以上结果表明,小鼠蛋白质的氧化损伤与气态甲醛的浓度有关,气态甲醛在较低浓度时,不引起小鼠蛋白质的氧化损伤,而在中等浓度时对小鼠心和肝的蛋白质氧化损伤作用显著,而对脑的蛋白质没有明显损伤作用.

关 键 词:甲醛  蛋白质氧化损伤  羰基含量  室内空气污染
文章编号:0253-2468(2005)06-0851-04
收稿时间:2004/9/10 0:00:00
修稿时间:2004年9月10日

Study on protein oxidative damage in mice induced by gaseous formaldehyde
DUAN Liju,ZHU Yan,HU Qinglian,LIU Yingshuai and YANG Yu.Study on protein oxidative damage in mice induced by gaseous formaldehyde[J].Acta Scientiae Circumstantiae,2005,25(6):851-854.
Authors:DUAN Liju  ZHU Yan  HU Qinglian  LIU Yingshuai and YANG Yu
Institution:College of Life Science, Central China Normal University, Wuhan 430079,College of Life Science, Central China Normal University, Wuhan 430079,College of Life Science, Central China Normal University, Wuhan 430079,College of Life Science, Central China Normal University, Wuhan 430079 and College of Life Science, Central China Normal University, Wuhan 430079
Abstract:In order to explore the degree of protein oxidative damage induced by gaseous formaldehyde in mice and its molecular reaction mechanism, the mice were treated with gaseous formaldehyde of different concentrations (0mg·m-3, 0.68mg.m-3 and 3 mg·m-3) for 72hours and the protein carbonyl content was measured by using spectrophotometric DNPH assay to reflect the degree of protein oxidative damage. The results showed that the protein carbonyl content in brain, heart and liver tissue of mice decreased in the group of 0.68 mg·m-3 (P<0.01, P<0.01, P<0.05), while it increased significantly in heart and liver tissue in the group of 3.0 mg·m-3 (P<0.01, P<0.01), but the protein carbonyl content in brain did not have notable difference compared with the control (P>0.05). These results indicated that the protein oxidative damage induced by gaseous formaldehyde depended on the concentration of gaseous formaldehyde. Low concentration formaldehyde might not induce protein oxidative damage, while medium concentration formaldehyde could cause protein oxidative damage in heart and liver tissue markedly, but it had little effect on protein of brain.
Keywords:formaldehyde  protein oxidative damage  carbonyl content  indoor air pollution
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