MBR和CAS工艺污泥在贫营养培养条件下的微生物群落结构研究

采用聚合酶链式-变性梯度凝胶电泳（PCR-DGGE）技术,研究了膜生物反应器（MBR）和传统活性污泥工艺（CAS）反应器中微生物在贫营养条件下的总细菌群落结构.结果表明,在培养过程中,污泥的微生物种群经历了一个比较明显的变化过程,且以CAS污泥微生物种群的变化更为明显,演替过程中既有原始优势种群的消亡,又有新的优势种群...

Microbial community structure of sludge in MBR and CAS under oligotrophic environment

Total microbial community structure in membrane bio-reactor (MBR) and conventional activated sludge (CAS) processes operated under oligotrophic environment was investigated using PCR-denaturing gradient gel electrophoresis (PCR-DGGE). Investigation results show that microbial population of the two sluges had significant change during cultivation process, especially for the sludge in CAS process. In the evolvement of microbial population, the original dominant species was replaced by new species gradually and the original dominant species was even died out from the system. The state of the dominant populations was in a dynamic change. Different sludge sources, influent water quality and operating conditions of the two systems lead to the differences of microbial community structure in activated sludge. The main dominant bacteria in the two sludges are uncultured bacterium, Arcobacter, Pseudomonas, Bacillus and gamma proteobacterium bacteria. Moreover, there also exists some pathogenic microorganisms, such as Arcobacter, Serratia and Klebsiella species.