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| 利用绿色荧光蛋白标记革兰氏阴性细菌的研究 | |
| 引用本文： | 崔长征,沈萍,张甲耀,冯耀宇,林匡飞.利用绿色荧光蛋白标记革兰氏阴性细菌的研究[J].环境科学学报,2011,31(2):276-282. |
| 作者姓名： | 崔长征 沈萍 张甲耀 冯耀宇 林匡飞 |
| 作者单位： | 1. 华东理工大学资源与环境工程学院国家环境保护化工过程环境风险评价与控制重点实验室,上海,200237 2. 武汉大学生命科学学院,武汉,430072 3. 武汉大学资源与环境科学学院,武汉,430072 |
| 摘 要： | 构建了具有不同抗性且能够组成型表达绿色荧光蛋白的一系列转座子质粒pTnMod-OCm-G、pTnMod-OTc-G、pTnMod-OKm3-G和pTnMod-OGm-G,并通过三亲本杂交的方法,成功地将荧光蛋白基因分别插入到多环芳烃降解菌株Sphingomonas sp.12A和Pseudomonas sp.12B的基... |
| 关 键 词： | 绿色荧光蛋白 组成型表达 转座子 生物降解 |
| 收稿时间： | 2010/5/26 0:00:00 |
| 修稿时间： | 8/22/2010 4:57:23 PM |
Labeling gram-negative bacteria using green fluorescent protein |
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| CUI Changzheng,SHEN Ping,ZHANG Jiayao,FENG Yaoyu and LIN Kuangfei.Labeling gram-negative bacteria using green fluorescent protein[J].Acta Scientiae Circumstantiae,2011,31(2):276-282. | |
| Authors: | CUI Changzheng SHEN Ping ZHANG Jiayao FENG Yaoyu and LIN Kuangfei |
| Institution: | State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237,College of Life Science, Wuhan University, Wuhan 430072,College of Resource and Environmental Science, Wuhan University, Wuhan 430072,State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237 and State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, School of Resources and Environmental Engineering, East China University of Science and Technology, Shanghai 200237 |
| Abstract: | In this study, four transposable plasmids were constructed, pTnMod-OCm-G, pTnMod-OTc-G, pTnMod-OKm3-G and pTnMod-OGm-G, which can constitutively express green fluorescent protein. Via triparental mating, GFP gene was inserted into genomes of Sphingomonas sp. 12A and Pseudomonas sp. 12B, two polycyclic aromatic hydrocarbons-degrading bacterial strains, respectively. The transformants which can degrade polycyclic aromatic hydrocarbons, can constitutively express GFP without the presence of antibiotics through many generations of propagation. The transposons can be transferred to other gram negative bacteria and expressed stably. Therefore, they have the potential to be used in research on bacterial survival and ecological safety in contaminated environments. |
| Keywords: | green fluorescent protein constitutively express transposons bidegradation |
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