高效石油降解菌的筛选鉴定及修复能力研究

为了得到高效的石油降解菌,本研究利用以柴油为唯一碳源的培养基从山东胜利油田、新疆克拉玛依油田和陕西长庆油田3处的石油污染土壤中富集纯化出3株高效的石油降解菌,分别命名为WTS、Z3-P和H4-1.测试结果显示,经过10d的降解实验,这3株降解菌对柴油的降解率均达到60%以上,降解效果良好,其中,WTS的降解效率最高,达到75%;用这3株菌进行污染土壤的修复实验,污染土壤中石油烃降解半衰期为30d左右,为自然情况下的1/4左右.对石油降解菌进行生理生化反应鉴定以及细菌16S rRNA鉴定结果表明,WTS是柠檬酸杆菌(Citrobacter sp.),H4-1是木糖氧化产碱菌(Alcaligenes xylosoxydans),Z3-P为芽孢杆菌(Bacillus sp.).

Isolation and identification of petroleum degrading bacteria and their remediation ability

In this paper, three strains of bacteria, WTS, Z3-P and H4-1, were isolated from petroleum-contaminated soils sampled from oil fields in Shandong Shengli, Xinjiang Karamay and Shaanxi Changqing. The ability of the strains to degrade diesel was examined, and after 10days the results showed that each strain was able to remove more than 60% of the total petroleum hydrocarbons, the WTS was proved that it had the most powerful ability, and the removal was 75%. When the strains were applied to real petroleum\|contaminated soils, the degradation half-life of petroleum was about 30 days, and reduced to less than one fourth of that under natural conditions. Physiological and biochemical analyses and 16S rRNA sequencing were conducted to identify the three strains. The results showed that WTS, H4-1 and Z3-P belong to Citrobacter sp., Alcaligenes xylosoxydans, and Bacillus sp., respectively.