| FISH方法解析大辽河入海口沉积物中微生物的垂直分布特征 |
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| 引用本文： | 全向春,王育来,何孟常,林春野,杨志峰.FISH方法解析大辽河入海口沉积物中微生物的垂直分布特征[J].环境科学学报,2009,29(7):1502-1509. |
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| 作者姓名： | 全向春 王育来 何孟常 林春野 杨志峰 |
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| 作者单位： | 北京师范大学环境学院/水环境模拟国家重点实验室,北京,100875 |
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| 基金项目： | 国家重点基础研究发展规划(973计划) |
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| 摘 要： | 取大辽河人海口柱状沉积物,通过寡核苷酸荧光探针原位杂交(FISH)方法分析了沉积物中微生物数量及种群的垂直分布特征,并考察了沉积物理化特性对微生物丰度的影响.结果表明,用5-(4,6-二氯三嗪基)氨基荧光素(简称DTAF)染料染色及EUB338探针杂交检测出的微生物细胞总数及细菌数量随沉积物深度呈现相同变化趋势.在沉积物表层(0-5cm)和中间层(23.5-29.5 cm)数量相对较高.细胞总数为7.9×108-20.1×108cell·cm-3,细菌数量为5.1×108-14.4×108个·cm-3,细菌检出率平均值为75.4%.用ALF1b、BET42a和GAM42a探针检测出α-、β和γ-变形杆菌在柱状沉积物中的普遍存在,α-、β和γ-变形杆菌检出量占细胞总数的25.O%-65.6%,其中以γ-变形杆菌为优势菌,其检出率为9.8%-40.8%.用ARCH915探针检测出的古细菌数量占细胞总量的1.0%-11.8%.总体看来,沉积物中微生物数量与沉积物的粘土含量呈正相关,在一定程度上受总有机碳影响,但与汞含量关系不明显.
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| 关 键 词： | 大辽河 沉积物 微生物 荧光原位杂交 |
| 收稿时间： | 2008/8/19 0:00:00 |
| 修稿时间： | 2008/12/18 0:00:00 |
Characterization of microbial vertical distribution in sediment from the estuary of the Daliao River by the FISH method |
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| QUAN Xiangchun,WANG Yulai,HE Mengchang,LIN Chunye and YANG Zhifeng.Characterization of microbial vertical distribution in sediment from the estuary of the Daliao River by the FISH method[J].Acta Scientiae Circumstantiae,2009,29(7):1502-1509. |
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| Authors: | QUAN Xiangchun WANG Yulai HE Mengchang LIN Chunye and YANG Zhifeng |
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| Institution: | State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing 100875,State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing 100875,State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing 100875,State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing 100875 and State Key Laboratory of Water Environmental Simulation, School of Environment, Beijing Normal University, Beijing 100875 |
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| Abstract: | |
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| Keywords: | the Daliao River sediment microorganism Fluorescence in Situ Hybridization |
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