尿中1-羟基芘的研究——人体接触多环芳烃的指标

对尿中1-羟基芘的分离和测定方法进行了研究和改进。10ml尿样经酶水解后,用反相高压液相色谱-荧光检测器分析,一小时左右即可得到定量结果,1-羟基芘的检出限为0.047ng。测定了对照组、交通警和焦炉工人尿中1-羟基芘的含量。讨论了人尿中1-羟基芘的量和接触多环芳烃的关系,认为尿中的1-羟基芘可做为人体接触空气中多环芳烃的一个指标。

URINARY 1-HYDROXYPYRENE AS AN INDICATOR OF EXPOSURE TO POLYNUCLEAR AROMATIC HYDROCARBONS

A procedure for isolation and determination of 1-hydroxypyrene in urine using reversed High pressure chromatography-fluorescence detector was modified and the method needs only 10ml of urine sample and the detection limit is 0.047 ng for 1-hydroxypyrene. The method was applied to measure the urine samples from the coke oven workers, the traffic policemen and a control group. The results showed that the highest content of -urinary 1-hydroxypyrene was found in the samples from the coke oven workers, followed by the traffic policemen. There was no significant difference between smokers and non-smokers in the control group. The relation between the urinary 1-hydroxypyrene of the coke oven workers and air content of polynuclear aromatic hydrocarbons at their working area was discussed and it was suggested that the former might be an indicator of the exposure to polynuclear aromatic hydrocarbons.