| 热处理重组质粒DNA的复性及其在水环境中的降解特性 |
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| 引用本文： | 李梦南,郑广宏,王磊,付小花,肖伟,乐毅全,任大明.热处理重组质粒DNA的复性及其在水环境中的降解特性[J].环境科学学报,2009,29(9):1800-1805. |
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| 作者姓名： | 李梦南 郑广宏 王磊 付小花 肖伟 乐毅全 任大明 |
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| 作者单位： | 1. 同济大学污染控制与资源化研究国家重点实验室,上海,200092
2. 复旦大学遗传学研究所遗传工程国家重点实验室,上海,200433 |
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| 基金项目： | 国家自然科学基金(No.40571145,40871217)~~ |
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| 摘 要： | 为了考察热处理后的重组DNA进入水环境后可能存在的环境风险,以pET-28b质粒为材料,以质粒相对转化效率为指标,考察了热变性重组质粒DNA的复性可能性,并在此基础上构建了人工模拟水环境系统,研究了热变性质粒DNA在水环境中的降解速率和影响因素.结果表明,热变性pET-28b质粒经过30min后,其转移活性可以得到恢复,在4～37℃之间,温度越高越有利于热变性质粒的复性.热处理过程中未被降解的质粒DNA进入水环境后,在pH值为7、8时降解速率相对较慢,在pH值为5、6、9的条件下,降解较快,但在任何pH下,1.0h后仍存在未降解的质粒DNA.水环境中的NaCl对热处理质粒DNA有一定的保护作用,而且这种作用是随着NaCl质量分数的提高而增强.进入水环境中的热变性DNA有足够的时间复性,从而可能发生基因转移.因此,这些热处理的质粒DNA进入环境后理论上存在一定的生态风险.
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| 关 键 词： | 热处理 质粒DNA 水环境 复性 转化 降解 |
| 收稿时间： | 12/8/2008 1:53:17 PM |
| 修稿时间： | 4/27/2009 9:56:03 AM |
Renaturation and degradation of thermo-treated waste plasmid DNA in an aquatic-environment |
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| LI Mengnan,ZHENG Guanghong,WANG Lei,FU Xiaohu,XIAO Wei,LE Yiquan and REN Daming.Renaturation and degradation of thermo-treated waste plasmid DNA in an aquatic-environment[J].Acta Scientiae Circumstantiae,2009,29(9):1800-1805. |
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| Authors: | LI Mengnan ZHENG Guanghong WANG Lei FU Xiaohu XIAO Wei LE Yiquan and REN Daming |
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| Institution: | State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092,State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092,State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092,State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092,State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433,State Key Laboratory of Pollution Control and Resource Reuse, Tongji University, Shanghai 200092 and State Key Laboratory of Genetic Engineering, Fudan University, Shanghai 200433 |
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| Abstract: | Plasmid pET-28b was used as a test plasmid to investigate the potential environmental risk caused by thermo-treated recombinant DNA. Firstly,plasmid DNA transformation was performed to evaluate the re-naturation efficiency of the thermo-treated plasmid DNA,and then simulated aquatic environments were constructed to determine the persistence of un-decayed plasmid DNAs in the aquatic environment after thermo-treatment. The results showed that the transforming activity of the thermo-treated plasmid DNA could b... |
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| Keywords: | thermo-treatment plasmid DNA stimulated aquatic environment renaturation transformation degradation |
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