| 植物酶浓度对检测乐果灵敏度的影响研究 |
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| 引用本文： | 肖建军,华泽钊,徐斐,李保国.植物酶浓度对检测乐果灵敏度的影响研究[J].环境科学学报,2002,22(5):653-657. |
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| 作者姓名： | 肖建军 华泽钊 徐斐 李保国 |
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| 作者单位： | 上海理工大学,食品科学与工程研究所,上海,200093 |
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| 基金项目： | 国家自然科学基金会项目 (50 1 760 32 ) |
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| 摘 要： | 从小麦中提取植物酶，以农药乐果为抑制剂，采用分光光度法研究了用各种酶浓度小麦酯酶检测乐果的分辨率和灵敏度。研究显示，存在一最佳酶浓度，用此浓度的酶液检测乐果，分辨率和灵敏度最高。
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| 关 键 词： | 乐果 农药残留 植物酶浓度 灵敏度 比色法 |
| 文章编号： | 0253-2468(2002)-05-0653-05 |
| 收稿时间： | 2001/10/29 0:00:00 |
| 修稿时间： | 2001年10月29 |
Influence of plant esterase concentration on sensitivity of pesticide residue detection |
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| XIAO Jianjun,HUA Zezhao,XU Fei and LI Baoguo.Influence of plant esterase concentration on sensitivity of pesticide residue detection[J].Acta Scientiae Circumstantiae,2002,22(5):653-657. |
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| Authors: | XIAO Jianjun HUA Zezhao XU Fei and LI Baoguo |
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| Institution: | Institute of Food Science and Engineering, Shanghai University of Science and Technology, Shanghai 200093,Institute of Food Science and Engineering, Shanghai University of Science and Technology, Shanghai 200093,Institute of Food Science and Engineering, Shanghai University of Science and Technology, Shanghai 200093 and Institute of Food Science and Engineering, Shanghai University of Science and Technology, Shanghai 200093 |
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| Abstract: | Using the pesticide dimethoate as an inhibitor, the colorimetric measurement of the inhibitory effect of dimethoate on the plant esterase extracted from wheat was carried out. The relationship between resolution and sensitivity with enzyme concentration was assayed. The results indicated that there is an optimum enzyme concentration under witch the resolution is the highest and sensitivity is very high. |
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| Keywords: | pesticide residue plant esterase concentration sensitivity spectrophotometric method |
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