以玉米秸秆为底物的纤维素降解菌与产电菌联合产电的可行性

利用单室空气阴极微生物燃料电池（MFC）反应器，以玉米秸秆为底物，以本实验室筛选和保存的纤维素降解菌Chaetomium sp.和Bacillus sp.，以及纤维素降解混合菌PCS-S和H-C为秸秆降解的生物催化剂，探讨了以汽爆秸秆固体为底物进行微生物产电的可行性.结果表明，在MFC系统内，纤维素降解纯菌和混合菌均能使纤维素降解，但产生的电压很低（<90mV，1000Ω），升高温度（30～38.5℃）对电压输出无明显影响.单独以生活污水作为菌源不能直接降解秸秆产电.只有将H-C和生活污水（产电菌源）混合作为接种体，MFC才能获得较高的电压输出.此时得到的以汽爆秸秆固体作为底物时的最大功率密度为406mW·m-2，仅比葡萄糖作为底物时所得到的最大功率密度510 mW·m-2低20%.

Electricity generation from corn stover by cellulose degradation bacteria and exoelectrogenic bacteria

The possibility of direct electricity production from steam exploded com stover residue was studied in single chamber air-cathode microbial fuel cells ( MFCs) using two cellulose degrading bacteria ( Chaetomium sp. , Bacillus sp. ) and two cellulose degrading communities (PCS-S and H-C; stored in our laboratory) as biocatalysts. Both pure strains and mixed communities can decompose corn stover in MFCs, but little electricity ( < 90 mV , 1000Ω) was generated during this process. Increasing the temperature from 30 to 38. 5℃ did not increase voltage outputs. Using domestic wastewater solely as inoculum, electricity cannot be generated from degradation of com stover. Maximum voltage was observed in the MFC using H-C co-operated with the exoelectrogenic bacteria. The maximum power density from steam exploded com stover residue was 406 mW · m~(-2) , which was only 20% lower than the 510 mW·m~(-2) obtained using glucose as a substrate.