农药毒死蜱对小鼠脑细胞氧化损伤的研究

毒死蜱是一种高效、低毒、广谱的有机磷杀虫杀螨剂.为研究其对生物体的氧化损伤,以昆明小鼠为受试体,毒死蜱按3、6和12 mg·kg-13个剂量水平灌胃染毒小鼠7d,以脑组织匀浆测定活性氧(Reactive oxygen species,ROS)、还原型谷胱甘肽(Glutathione,GSH)和丙二醛(Malondialdehyde,MDA)的含量,以脑细胞测定DNA-蛋白质交联(DNA-protein Crosslink,DPC)系数.实验结果表明,随着毒死蜱染毒剂量的升高,ROS和MDA含量及DPC系数逐渐上升,GSH含量逐渐降低,各指标呈一定的剂量-效应关系.染毒剂量为6 mg·kg-1时,与对照组相比,DPC有显著差异(p＜0.05),MDA有极显著差异(P＜0.01)；染毒剂量为12 mg·kg-1时,与对照组相比,ROS和DPC有显著差异(p＜0.05),GSH和MDA有极显著差异(p＜0.01).说明较高剂量的毒死蜱可造成小鼠脑组织的氧化损伤和DNA-蛋白质交联作用增强.

Oxidative damage of mouse brain cells induced by pesticide chlorpyrifos

Chlorpyrifos is an efficient, low toxicity, broad-spectrum organophosphorus insecticide acaricide. This study aims at identifying the oxidative stress effects of chlorpyrifos on organisms. Kunming mice were orally administrated with chlorpyrifos daily for seven days at the concentration of 3, 6 and 12 mg·kg^-1, respectively. The contents of reactive oxygen species (ROS), glutathione (GSH) and malondialdehyde (MDA) in the brain homogenate and DNA-protein Crosslink (DPC) coefficients in the brain cells were measured to indicate the oxidative damages. The experimental results showed that the contents of ROS, MDA and DPC coefficients increased gradually while GSH content decreased with the increasing exposure dose, and all the biomarkers were in the exposure dose-response manner. When the exposure dose was at 6 mg·kg^-1, DPC coefficient was significantly higher than the control group (p<0.05), and MDA contents had extremely significant differences (p<0.01). In the higher dose groups (12 mg·kg^-1), ROS content and DPC coefficient indicated significant differences compared with the control group (p<0.05), and GSH and MDA contents had extremely significant differences (p<0.01). These experimental results demonstrated that chlorpyrifos can increase the oxidative stress and DNA-protein crosslinks in mouse brain at a higher dose.