| 一种分离纯化降解性质粒DNA的简便方法 |
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| 引用本文： | 王春生,简浩然,王志通.一种分离纯化降解性质粒DNA的简便方法[J].环境科学学报,1984,4(1):69-72. |
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| 作者姓名： | 王春生 简浩然 王志通 |
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| 作者单位： | 中国科学院武汉病毒研究所
(王春生，简浩然)，中国科学院武汉病毒研究所(王志通) |
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| 摘 要： | 控制微生物对某些环境污染物降解的质粒——降解性质粒,分子量较大,难于和染色体DNA分离。超螺旋质粒DNA在热变性后冷却即快速复性方面与染色体DNA不同。根据此性质,作者改进了McMaster的和Kado的方法,获得了良好效果。实验证明。经此法纯化的质粒具有转化活性,是研究化学污染物的降解性质粒的简便方法。
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| 收稿时间： | 1983/4/25 0:00:00 |
A RAPID METHOD FOR ISOLATION AND PURIFICATION OF DEGRADATIVE PLASM1D DNA |
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| Wang Chunsheng,Jian Haoran and Wang Zhitong.A RAPID METHOD FOR ISOLATION AND PURIFICATION OF DEGRADATIVE PLASM1D DNA[J].Acta Scientiae Circumstantiae,1984,4(1):69-72. |
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| Authors: | Wang Chunsheng Jian Haoran and Wang Zhitong |
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| Institution: | Wuhan Institute of Virology, Academia Sinica,Wuhan Institute of Virology, Academia Sinica and Wuhan Institute of Virology, Academia Sinica |
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| Abstract: | Degradative plasmids which contain some genomes characteristics of microbial degradation of some environmental pollutants are of large molecular weight and hence difficult to be isolated from chromosome. Unlike chromosomal DNA, plasmid is supercoiled in structure, which renatures rapidly after heat denaturation followed by quick cooling. Taking advantage of this kind of property, a modification of both McMaster's and Kado's methods has been devised and works successfully. Experimental results indicate that the plasmids isolated by this method retain the transformational effect. It seems to be a useful and rapid method to study plasmid on microbial degradation of recalcitrant pollutants |
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