| 分解纤维素的高温真菌筛选及其对烟杆的降解效果 |
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| 引用本文： | 张楠,刘东阳,杨兴明,徐阳春,沈其荣,黄启为.分解纤维素的高温真菌筛选及其对烟杆的降解效果[J].环境科学学报,2010,30(3):549-555. |
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| 作者姓名： | 张楠 刘东阳 杨兴明 徐阳春 沈其荣 黄启为 |
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| 作者单位： | 南京农业大学资源与环境科学学院,南京,210095 |
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| 基金项目： | 农业部农业公益性行业科研专项（No.200803031）；农业部948项目（No.2006-G62） |
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| 摘 要： | 为研制促进烟杆堆肥的微生物菌剂,进行了分解纤维素的高温真菌筛选.结果表明,从不同原料腐熟堆肥中筛选到4株降解纤维素的高温真菌,它们均能在以羧甲基纤维素钠(CMC-Na)或烟杆粉末为唯一碳源的培养基上生长.菌株在2～5d内可长满CMC-Na-刚果红平板和烟杆粉末培养基.菌株摇瓶培养时2d可达到产酶高峰,酶活(CMCase)超过10U.菌株对烟杆的降解效果较好,7d内的降解率最高可达42.2%,对烟杆中的纤维素、半纤维素和木质素的分解率分别可达52.7%、47.9%和37.6%.
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| 关 键 词： | 纤维素降解真菌 降解 烟杆 纤维素 半纤维素 木质素 |
| 收稿时间： | 2009/4/24 0:00:00 |
| 修稿时间： | 2009/10/19 0:00:00 |
Screening of thermophilic cellulose-decomposing fungi and their efficiency in decomposing tobacco stems |
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| ZHANG Nan,LIU Dongyang,YANG Xingming,XU Yangchun,SHEN Qirong and HUANG Qiwei.Screening of thermophilic cellulose-decomposing fungi and their efficiency in decomposing tobacco stems[J].Acta Scientiae Circumstantiae,2010,30(3):549-555. |
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| Authors: | ZHANG Nan LIU Dongyang YANG Xingming XU Yangchun SHEN Qirong and HUANG Qiwei |
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| Institution: | College of Resources and Environment Sciences, Nanjing Agriculture University, Nanjing 210095,College of Resources and Environment Sciences, Nanjing Agriculture University, Nanjing 210095,College of Resources and Environment Sciences, Nanjing Agriculture University, Nanjing 210095,College of Resources and Environment Sciences, Nanjing Agriculture University, Nanjing 210095,College of Resources and Environment Sciences, Nanjing Agriculture University, Nanjing 210095 and College of Resources and Environment Sciences, Nanjing Agriculture University, Nanjing 210095 |
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| Abstract: | Thermophilic cellulose-decomposing fungi were screened to develop microbial inoculums which could promote the composting of tobacco stems. Four strains of thermophilic cellulose-decomposing fungi were isolated from composts of different substrates, and they could use carboxymethyl cellulose Na (CMC-Na) or tobacco stem powder as sole carbon source. The strains could overgrow in CMC Na-Congo red medium and tobacco stem powder medium within 2~5 days. In liquid medium, the strains produced the highest CMCase activity after 2 days inoculation, which reached 10U. They could decompose tobacco stems, cellulose, hemicellulose and lignin in the tobacco stems by 42.2%, 52.7%, 47.9% and 37.6%, respectively, within 7 days. |
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| Keywords: | cellulose-decomposing fungi decompose tobscco stems cellulose hemicellulose lignin |
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