| Aureobasidium pullulans UVMU3-1产耐热性胞外漆酶活性物质培养基优化及其对染料脱色作用 |
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| 引用本文： | 孔丽,米晓春,耿圆圆,林超,王金鹏,李利,李炳学,张宁.Aureobasidium pullulans UVMU3-1产耐热性胞外漆酶活性物质培养基优化及其对染料脱色作用[J].环境科学学报,2015,35(12):3824-3831. |
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| 作者姓名： | 孔丽 米晓春 耿圆圆 林超 王金鹏 李利 李炳学 张宁 |
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| 作者单位： | 沈阳农业大学生物科学技术学院, 沈阳 110866,沈阳农业大学土地与环境学院土肥资源高效利用国家工程实验室, 沈阳 110866,沈阳农业大学生物科学技术学院, 沈阳 110866,沈阳农业大学生物科学技术学院, 沈阳 110866,沈阳农业大学生物科学技术学院, 沈阳 110866,沈阳农业大学土地与环境学院土肥资源高效利用国家工程实验室, 沈阳 110866,沈阳农业大学土地与环境学院土肥资源高效利用国家工程实验室, 沈阳 110866,沈阳农业大学生物科学技术学院, 沈阳 110866 |
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| 基金项目： | 国家自然科学基金(No.31271818);中国博士后科学基金(No.2012M510837);沈阳市科技创新专项(No.F13-143-3-00) |
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| 摘 要： | 对出芽短梗霉无色素突变株Aureobasidium pullulans UVMU3-1产耐热性胞外漆酶活性物质培养基进行优化,并研究其分子量大小、热稳定性及对三苯甲烷类染料孔雀石绿和结晶紫的脱色作用.结果表明:蔗糖、NaNO_3为UVMU3-1产耐热性胞外漆酶活性物质的最佳碳源和氮源,产酶优化培养基配方为:蔗糖66.5 g·L~(-1),NaNO_338.4 g·L~(-1),NaCl 1.08 g·L~(-1),酵母浸粉0.2 g·L~(-1),KH_2PO_41 g·L~(-1),pH=6.0.影响UVMU3-1产耐热性胞外漆酶活性物质的因素依次为:起始pH蔗糖NaClNaNO_3,培养7 d时产生最大酶活(94.81 U·L~(-1)),比对照提高3.85倍.其分子量小于1000 D,具有良好的热稳定性,煮沸30 min后高温酶活仍残留72.50%.粗酶液(7.5 U·L~(-1))与三苯甲烷类代表性染料孔雀石绿和结晶紫75℃反应5 min后,脱色率分别为76.3%、77.5%,反应35 min后脱色率分别达到96.3%、95.3%.
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| 关 键 词： | 出芽短梗霉 漆酶活性物质 耐热性 染料脱色 |
| 收稿时间： | 2015/1/11 0:00:00 |
| 修稿时间： | 2015/3/13 0:00:00 |
Optimization of culture conditions for thermostable extracellular laccase activity of Aureobasidium pullulans UVMU3-1 and its performance in dye decolorization |
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| KONG Li,MI Xiaochun,GENG Yuanyuan,LIN Chao,WANG Jinpeng,LI Li,LI Bingxue and ZHANG Ning.Optimization of culture conditions for thermostable extracellular laccase activity of Aureobasidium pullulans UVMU3-1 and its performance in dye decolorization[J].Acta Scientiae Circumstantiae,2015,35(12):3824-3831. |
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| Authors: | KONG Li MI Xiaochun GENG Yuanyuan LIN Chao WANG Jinpeng LI Li LI Bingxue and ZHANG Ning |
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| Institution: | College of Biological Science & Technology Shenyang Agricultural University, Shenyang 110866,National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang 110866,College of Biological Science & Technology Shenyang Agricultural University, Shenyang 110866,College of Biological Science & Technology Shenyang Agricultural University, Shenyang 110866,College of Biological Science & Technology Shenyang Agricultural University, Shenyang 110866,National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang 110866,National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang 110866 and College of Biological Science & Technology Shenyang Agricultural University, Shenyang 110866 |
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| Abstract: | |
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| Keywords: | Aureobasidium pullulans laccase activity substance thermostable dye decolorization |
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