| 一株丁草胺降解菌的分离鉴定及培养条件优化 |
| |
| 引用本文： | 李春艳,李艳春,成小松,肖晶,熊明华,潘俊波.一株丁草胺降解菌的分离鉴定及培养条件优化[J].环境科学学报,2010,30(2):347-353. |
| |
| 作者姓名： | 李春艳 李艳春 成小松 肖晶 熊明华 潘俊波 |
| |
| 作者单位： | 1. 东北农业大学资源与环境学院,哈尔滨,150030
2. 哈尔滨医科大学第一临床医学院,哈尔滨,150001 |
| |
| 基金项目： | 东北农业大学创新团队基金(No. CXT003-1-3)；黑龙江省教育厅科学技术研究项目(No.11541015) |
| |
| 摘 要： | 利用富集培养技术从长期施用丁草胺的稻田土壤中分离得到1株能够降解丁草胺的细菌,标记为LYC-2.经形态特征、生理生化特征和16SrDNA序列分析,将该菌株鉴定为钩杆菌属(Ancylobacter sp.).同时,为探索菌株LYC-2的生长特性和最佳培养条件,利用正交试验设计法考察了接种量、温度、pH值3个因素对菌株LYC-2生长的影响.结果表明,菌株LYC-2的最适生长温度为35℃,最适pH值为7.5.当接种量为6%(体积分数,下同)时,该菌株在含100mg.L-1的丁草胺无机盐基础培养液中培养5d后,可使丁草胺降解率达90.85%以上.
|
| 关 键 词： | 丁草胺 Ancylobacter sp. 分离鉴定 生长特性 培养条件 正交试验 |
| 收稿时间： | 3/18/2009 1:37:07 PM |
| 修稿时间： | 6/22/2009 4:11:18 PM |
Isolation and identification of a butachlor degrading bacterium and optimization of its growth conditions |
| |
| LI Chunyan,LI Yanchun,CHENG Xiaosong,XIAO Jing,XIONG Minghua and PAN Junbo.Isolation and identification of a butachlor degrading bacterium and optimization of its growth conditions[J].Acta Scientiae Circumstantiae,2010,30(2):347-353. |
| |
| Authors: | LI Chunyan LI Yanchun CHENG Xiaosong XIAO Jing XIONG Minghua and PAN Junbo |
| |
| Institution: | College of Resource and Environment, Northeast Agricultural University, Harbin 150030,College of Resource and Environment, Northeast Agricultural University, Harbin 150030,First Clinical medical College, Harbin Medical University, Harbin 150001,College of Resource and Environment, Northeast Agricultural University, Harbin 150030,College of Resource and Environment, Northeast Agricultural University, Harbin 150030 and College of Resource and Environment, Northeast Agricultural University, Harbin 150030 |
| |
| Abstract: | A butachlor-degrading strain LYC-2 was isolated from a paddy soil sample by enrichment culture. According to morphologic and physioblochemical characteristics as well as 16S rDNA sequence analysis, strain LYC-2 was identified as an Ancylobacter sp. Effects of inoculation rate (V/V), temperature and pH on the growth of the butachlor-degrading strain were examined using an orthogonal experiment. The results showed that the optimal inoculation rate was 6% (V/V) in mineral medium containing 100 mg·L~(-1) butachlor, at 35℃ and pH 7.5. Under these conditions, more than 90.85% butachlor was degraded by LYC-2 in 5 days. |
| |
| Keywords: | butachlor Ancylobacter sp isolation and identification growth characteristics cultural conditions orthogonal experiment |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《环境科学学报》浏览原始摘要信息 |
| 点击此处可从《环境科学学报》下载免费的PDF全文 |
|
