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| 混合菌对原油的降解及其降解性能的研究 | |
| 引用本文： | 何丽媛,党志,唐霞,卢桂宁,郭楚玲,吴平霄,刘从强.混合菌对原油的降解及其降解性能的研究[J].环境科学学报,2010,30(6):1220-1227. |
| 作者姓名： | 何丽媛 党志 唐霞 卢桂宁 郭楚玲 吴平霄 刘从强 |
| 作者单位： | 1. 华南理工大学环境科学与工程学院,广州,510006 2. 华南理工大学环境科学与工程学院,广州,510006;工业聚集区污染控制与生态修复教育部重点实验室,广州,510006 3. 中国科学院地球化学研究所,环境地球化学国家重点实验室,贵阳,550002 |
| 基金项目： | 广东省自然科学基金研究团队项目（No. 9351064101000001）；广东省自然科学基金重点项目（No. 05103552）；广东省科技计划项目（No. 2007A050100023）；环境地球化学国家重点实验室开放基金 |
| 摘 要： | 从污染土壤中分离筛选到四株石油组分降解菌被用于组建降解原油的混合菌体系.石油组分降解菌包括:烷烃降解菌洋葱伯克霍尔德氏菌(Burkholderia cepacia)GS3C、菲降解菌鞘氨醇单胞菌(Sphingomonas sp.)GY2B、芘降解菌GP3(假单胞菌(Pseudomonas sp.)GP3A和伯克菌科的菌株(Pandoraea pnomenusa)GP3B).气相色谱-质谱联用法(GC-MS)用于对原油降解性能的测定,并对原油组分的降解情况进行详细分析.通过对不同菌株的混合培养比较,得到降解原油的最佳组合G8(GS3C+GY2B+GP3B),培养5d后使初始原油浓度为2000mg·mL-1的总去除率达到69.20%,并且对烷烃类和芳烃类化合物都表现出较强的降解能力.混合菌G8对原油的总去除率比单菌提高了近30%,其最适生长条件为:温度为30℃,初始pH值为7,接种量为4%. |
| 关 键 词： | 混合菌 原油 生物降解 GC-MS |
| 收稿时间： | 2009/10/17 0:00:00 |
| 修稿时间： | 12/9/2009 1:40:10 PM |
Biodegradation characteristics of crude oil by mixed bacterial strains |
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| HE Liyuan,DANG Zhi,TANG Xi,LU Guining,GUO Chuling,WU Pingxiao and LIU Congqiang.Biodegradation characteristics of crude oil by mixed bacterial strains[J].Acta Scientiae Circumstantiae,2010,30(6):1220-1227. | |
| Authors: | HE Liyuan DANG Zhi TANG Xi LU Guining GUO Chuling WU Pingxiao and LIU Congqiang |
| Institution: | School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006,1. School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006; 2. The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006,School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006,School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006,1. School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006; 2. The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006,1. School of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006; 2. The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006 and The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangzhou 510006 |
| Abstract: | |
| Keywords: | mixed strains crude oil biodegradation GC-MS |
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