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| 利用PCR-DGGE溯源典型农村塘坝饮用水中的污染 | |
| 引用本文： | 冯广达,邓名荣,郭俊,王东东,朱红惠,张曦,梁浩亮,朱昌雄.利用PCR-DGGE溯源典型农村塘坝饮用水中的污染[J].环境科学学报,2011,31(3):468-475. |
| 作者姓名： | 冯广达 邓名荣 郭俊 王东东 朱红惠 张曦 梁浩亮 朱昌雄 |
| 作者单位： | 1. 广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广州,510070 2. 广东省微生物研究所,广东省菌种保藏与应用重点实验室,广东省微生物应用新技术公共实验室,广州,510070;中国农业科学院农业环境与可持续发展研究所,北京,100081 3. 惠州市水产业科学技术研究所,惠州,516007 4. 中国农业科学院农业环境与可持续发展研究所,北京,100081 |
| 基金项目： | 国家科技重大专项“水体污染控制与治理” 资助项目(No. 2008ZX07425-002) |
| 摘 要： | 以华南地区典型农村塘坝饮用水为研究对象,采用基于大肠杆菌的两种特异性基因(β-D-葡萄糖苷酶编码基因uidA和膜外周磷通道蛋白编码基因phoE)的群体差异分析(PCR-DGGE),对饮用水中的污染情况进行了溯源研究.结果表明,采用富集培养的混合菌体DNA作为模板扩增大肠杆菌特异性基因更加实用.uidA和phoE的引物均... |
| 关 键 词： | 微生物溯源 大肠杆菌 PCR-DGGE β-D-葡萄糖苷酶编码基因(uidA) 膜外周磷通道蛋白编码基因(phoE) |
| 收稿时间： | 6/17/2010 9:47:49 AM |
| 修稿时间： | 2010/8/10 0:00:00 |
Microbial source tracking of pollution in typical rural pond-drinking water using PCR-DGGE |
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| FENG Guangd,DENG Mingrong,GUO Jun,WANG Dongdong,ZHU Honghui,ZHANG Xi,LIANG Haoliang and ZHU Changxiong.Microbial source tracking of pollution in typical rural pond-drinking water using PCR-DGGE[J].Acta Scientiae Circumstantiae,2011,31(3):468-475. | |
| Authors: | FENG Guangd DENG Mingrong GUO Jun WANG Dongdong ZHU Honghui ZHANG Xi LIANG Haoliang and ZHU Changxiong |
| Institution: | Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070,Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070,Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070,Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070,Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070,1. Guangdong Institute of Microbiology, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou 510070; 2. Institute of Agricultural Environment and Sustainable Deve,Huizhou Fishery Institute of Science and Technology, Huizhou 516007 and Institute of Agricultural Environment and Sustainable Development, Chinese Academy of Agricultural Sciences, Beijing 100081 |
| Abstract: | Taking the rural pond-drinking water in south China as the object, microbial source tracking of water pollution was done using PCR-DGGE to analyze the differences in Escherichia coli communities based on two specific genes (the beta-D-glucuronidase coding gene uidA and the outer membrane phosphoporin protein coding gene phoE). According to our results, it is more practical to take all the cultured microbes DNA as templates in PCR. The primers of the gene uidA and phoE are of high specificity. Analysis of the two specific gene sequences in GenBank showed that the average degree of variation of phoE was about twice that of uidA. PCR-DGGE based on phoE could exactly reflect the difference and connection among the samples and be more adapted to microbial source tracking than uidA. The pond-drinking water and its surrounding water were polluted with non-point pollution and breeding waste might be the main source of pollution. |
| Keywords: | microbial source tracking Escherichia coli PCR-DGGE beta-D-glucuronidase coding gene (uidA) outer membrane phosphoporin protein coding gene (phoE) |
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