| 基于叶绿素荧光成像技术的藻毒性检测法的建立及在环境监测中的应用 |
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| 引用本文： | 郑凯,马晓妍,郝丽伟,王晓昌,金鹏康,杨文博.基于叶绿素荧光成像技术的藻毒性检测法的建立及在环境监测中的应用[J].环境科学学报,2019,39(3):768-773. |
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| 作者姓名： | 郑凯 马晓妍 郝丽伟 王晓昌 金鹏康 杨文博 |
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| 作者单位： | 西安建筑科技大学,西北水资源与环境生态教育部重点实验室,国家城市非传统水资源开发利用国际科技合作基地,陕西省环境工程重点实验室,西安710055;西安建筑科技大学,西北水资源与环境生态教育部重点实验室,国家城市非传统水资源开发利用国际科技合作基地,陕西省环境工程重点实验室,西安710055;西安建筑科技大学,西北水资源与环境生态教育部重点实验室,国家城市非传统水资源开发利用国际科技合作基地,陕西省环境工程重点实验室,西安710055;西安建筑科技大学,西北水资源与环境生态教育部重点实验室,国家城市非传统水资源开发利用国际科技合作基地,陕西省环境工程重点实验室,西安710055;西安建筑科技大学,西北水资源与环境生态教育部重点实验室,国家城市非传统水资源开发利用国际科技合作基地,陕西省环境工程重点实验室,西安710055;西安建筑科技大学,西北水资源与环境生态教育部重点实验室,国家城市非传统水资源开发利用国际科技合作基地,陕西省环境工程重点实验室,西安710055 |
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| 基金项目： | 国家自然科学基金（No.51508449，51778522）；陕西省教育厅科研计划项目（No.18JK0468） |
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| 摘 要： | 应用新型大面积调制叶绿素荧光成像系统(Maxi-Imaging-PAM),以蛋白核小球藻作为指示生物,以典型的光合作用抑制剂敌草隆和莠灭净为测试物质,建立了微孔板式藻毒性检测方法,确定了实验参数对结果的影响,并将该方法应用于检测石油废水的藻毒性.结果表明,最适实验条件为:藻细胞的暴露浓度在3.45×10~6～5.28×10~6 cell·mL~(-1)范围内,暴露时间为2 h时,毒性检测最灵敏,获得的敌草隆和莠灭净的半最大效应浓度(EC_(50))分别为(4.33±0.38)μg·L~(-1)和(3.87±0.27)μg·L~(-1).将该方法成功应用于某石油废水的藻毒性检测,确定其EC_(50)值为1.03%±0.07%(稀释百分数),相当于414.96μg·L~(-1)的敌草隆的毒性.本研究建立的微孔板式藻毒性检测方法具有灵敏度高、所需样品量少、可大批量测试等优点,对毒理学发展具有重要意义.
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| 关 键 词： | 叶绿素荧光成像技术 藻毒性 敌草隆 莠灭净 荧光量子产率 |
| 收稿时间： | 2018/8/31 0:00:00 |
| 修稿时间： | 2018/11/11 0:00:00 |
The establishment of algal toxicity detection method based on chlorophyll fluorescence imaging and its application in environmental monitoring |
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| ZHENG Kai,MA Xiaoyan,HAO Liwei,WANG Xiaochang,JIN Pengkang and YANG Wenbo.The establishment of algal toxicity detection method based on chlorophyll fluorescence imaging and its application in environmental monitoring[J].Acta Scientiae Circumstantiae,2019,39(3):768-773. |
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| Authors: | ZHENG Kai MA Xiaoyan HAO Liwei WANG Xiaochang JIN Pengkang and YANG Wenbo |
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| Institution: | Key Laboratory of Northwest Water Resources and Environmental Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shaanxi Province, Xi''an University of Architecture and Technology, Xi''an 710055,Key Laboratory of Northwest Water Resources and Environmental Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shaanxi Province, Xi''an University of Architecture and Technology, Xi''an 710055,Key Laboratory of Northwest Water Resources and Environmental Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shaanxi Province, Xi''an University of Architecture and Technology, Xi''an 710055,Key Laboratory of Northwest Water Resources and Environmental Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shaanxi Province, Xi''an University of Architecture and Technology, Xi''an 710055,Key Laboratory of Northwest Water Resources and Environmental Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shaanxi Province, Xi''an University of Architecture and Technology, Xi''an 710055 and Key Laboratory of Northwest Water Resources and Environmental Ecology, Ministry of Education, International Science & Technology Cooperation Center for Urban Alternative Water Resources Development, Key Lab of Environmental Engineering, Shaanxi Province, Xi''an University of Architecture and Technology, Xi''an 710055 |
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| Abstract: | |
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| Keywords: | chlorophyll fluorescence imaging technology algae toxicity diuron ametryn fluorescence quantum yield |
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