PCR-DGGE技术分析倒置A~2/O工艺处理染织废水中的微生物区系

基于16S rDNA的PCR-DGGE(polymerase chain reaction-denaturing gradient gel electrophoresis)技术研究了倒置A2/O(anoxic-anaerobic-aerobic)工艺处理染织废水过程中的微生物群落结构.从兼氧、厌氧和好氧区共取6个不同处理阶段的样品,测量了不同处理阶段样品的COD、pH、NH4+-N、H2S、总磷(TP)、污泥含量(TS)、色度及其脱除率,并分析了这些指标的变化规律.结果显示,COD在处理后期降到了145mg·L-1,NH4+-N的脱除率可达到85%,硫化氢的去除率达到了90%以上,磷的脱除率可达80%以上,TS处于逐渐升高的趋势,色度的脱除率达到65%.DGGE图谱显示,好氧处理阶段的微生物多样性明显比兼氧处理和厌氧处理的阶段的要丰富.

Microbial community structure analysis in activated sludge of dyeing-weaving wastewater treated with anoxic-anaerobic-aerobic process by PCR-DGGE

The microbial community structure in activated sludge of dyeing-weaving wastewater, treated by an anoxic-anaerobic-aerobic process, was studied by nested polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) based on 16S rDNA sequences. Activated sludge samples were collected from anoxic,anacrobic and aerobic conditions at 6 different treatment periods and COD ,pH, NH_4~+-N, H_2 S,TP、TS (tatal solids)、color and their removal rates at different times were monitored. The results showed that the value of COD decreased to 145 mg·L~(-1); the removal effieiencies of NH_4~+ -N, H_2S and P were above 85% ,90% and 80% respectively; while TS basically increased and the color removal rate was about 65%. The DGGE map showed that the microbial community was more abundant in the aerobic treatment periods than in the anaerobic and anoxic treatment periods.