硒对镰刀菌毒素毒性抑制作用的初步研究

用动物试验确定硒(Ⅳ)对镰刀菌中一种尖孢镰刀菌(Fusarium oxysporum)毒素的毒性有抑制作用。用人胚软骨细胞实验也初步观察到相似的结果,对该菌属代谢物之一镰刀菌酸与硒的化学作用进行了初步探讨。

INHIBITORY EFFECT ON THE TOXICITY OF FUSARIUM TOXIN BY SELENIUM

The inhibitory effect of selenium on the toxicity ot fusarium toxin which might be one of the factors causing Kaschin-Beck disease was studied.The strain of F. oxysporum NF 031 isolated from corn at Kaschin-Beck disease region was inoculated on sterilized maize meal and incubated at 28°C for 15 days. The toxin was extracted by CH₂Cl₂ and suspended in pure peanut oil. The tested mice were divided into three groups. Both control group injected with pure peanut oil of 0.5 ml and the second group injected with 0.5 ml oil containing toxin were fed with tap water. The third group was fed with water containing selemium for 2 days prior to toxin oil injection. All the mice in the second group died within one day whereas those in the other two groups were still alive after 20 days indicating that selenium might be a detoxicater of the toxin.Using human embryonic cartilage cell, the damage to the cell by water extractable F. oxysporum toxin was studied. After two days incubation the control group was continuously cultured in normol culture fluid and the test groups were incubated with the solution containing toxin alone, selenium alone, or toxin and selenium. All experiments were carried out at 37°C for two days. A majority of the cells died when the culture solution containing toxin alone and the addition of selenium decreased the toxicity of toxin.