Trametes sp.SQ01和Chaetomium sp.R01混合培养对四种染料的脱色

利用本实验室新构建的白腐菌Trametes sp.SQ01和毛壳菌Chaetomium sp.R01混合培养体系,对刚果红、酸性红、橙黄G和溴酚蓝4种染料进行了脱色研究.结果表明,SQ01与R01混合培养所产生的锰过氧化物酶(MnP)酶活比菌株SQ01单独培养时提高了约5.5倍.菌株R01的接种量、接种时间对混合培养中...

Decolorization of four dyes by a mixed culture of Trametes sp. SQ01 and Chaetomium sp. R01

Four dyes including Congo red, acid red, orange G and bromphenol blue were decolorized by a mixed fungal culture of Trametes sp. SQ01 and Chaetomium sp. R01, which were newly developed in our laboratory.The results indicated that MnP activity in the mixed culture of strain SQ01 and R01 increased approximately 5.5 times over that obtained from a pure culture of SQ01.The amount and timing of strain R01 had a marked effect on MnP production by SQ01.If the inoculum of strain R01 was too high or too low, the MnP activity decreased in the mixed culture.As the inoculation time of strain R01 was delayed, MnP activity was gradually reduced.MnP production was inhibited by about 40%~75% by the four dyes in mixed culture of SQ01 and R01.The decolorization rates of mixed culture of strain SQ01 and R01 were increased by 20%~50% compared to that of the monoculture of SQ01.After 3 days, 75%~94% of four dyes were decolorized by mixed culture of SQ01 and R01,while the decolorization rate was only about 41%~75% by strain SQ01 cultured alone.The time-point of dye addition remarkably influenced the efficiency of decolorization by the mixed culture.The decolorization rates of dyes added to the medium after 4 days of mixed cultivation were significantly higher as compared to dyes added at the outset of mixed cultivation.In addition to biodegradation,biosorption also played an important role in the decolorization process.The biosorption of orange G,bromphenol blue and acid red accounted for 2%,5% and 15% of the total color removal,respectively.However,the biosorption rate of congo red reached up to 60%.To date,this is the first report of decolorizing dyes by mixed fungal cultures. The high efficiency of the decolorization ability indicates that the mixed culture has broad application prospects in dye decolorization treatment.