聚乙烯醇降解酶产生菌发酵培养基响应面法优化研究

为了提高假单胞菌Pseudomonas.sp XT11-Z90S产聚乙烯醇降解酶的能力,用Plackett-Buman实验和响应面分析实验对影响产聚乙烯醇降解酶的9个因素,即发酵培养基的9种组成成分进行了研究,并探讨了影响产酶的主要因素和培养基的最佳组成.结果表明,影响产聚乙烯醇降解酶的3个重要组成为葡萄糖、酵母膏和硫酸铵,其最适浓度分别为0.64、0.22和1.45g.L-1.在最佳浓度和组成条件下,聚乙烯醇降解酶最高酶活为13.5U.mL-1,与优化前的12.1U.mL-1相比,提高了11.6%,且其降解率6h时可达到35.5%.

Optimization of the fermentation medium for Pseudomonas strain producing a poly(vinyl alcohol)-degrading enzyme based on response surface methodology

Two level factorial designs of the Plackett-Burman type were constructed to optimize the fermentation medium components of a poly(vinyl alcohol)-degrading enzyme produced by strain Pseudomonas sp. XT11-Z90S. The results showed that the concentrations of glucose, yeast extract and (NH4)2SO4 greatly influence the activity of poly(vinyl alcohol)-degrading enzyme. The optimum concentrations of glucose, yeast extract and (NH4)2SO4, determined by response surface methodology, were 0.64, 0.22 and 1.45 g·L-1, respectively. Under the optimal conditions mentioned above, the poly(vinyl alcohol)-degrading enzyme activity is 13.5U·mL-1, which was increased by 11.6% compared with the value before optimization.